ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2011
Housing and husbandry of Xenopus laevis affect the quality of oocytes for heterologous expression studies.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- SLC12A ion transporter mutations in sporadic and familial human congenital hydrocephalus.Molecular genetics & genomic medicine · 2019Trial
- Proteoliposomes reconstituted with human aquaporin-1 reveal novel single-ion-channel properties.Biophysical reports · 2023Article
- Late-onset sensory-motor axonal neuropathy, a novel SLC12A6-related phenotype.Brain : a journal of neurology · 2023Article
- Morphological, acoustic and genetic identification of a reproducing population of the invasive African clawed frogZooKeys · 2023Article
- The teleost fish PepT1-type peptide transporters and their relationships with neutral and charged substrates.Frontiers in physiology · 2023Article
- Inhibition mechanism of NKCC1 involves the carboxyl terminus and long-range conformational coupling.Science advances · 2022Article
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters.The Journal of physiology · 2022Article
- SLC12A2 variants cause a neurodevelopmental disorder or cochleovestibular defect.Brain : a journal of neurology · 2020Article
- Article
- Fluorescence lifetime imaging reveals heterogeneous functional distribution of eGFP expressed in Xenopus oocytes.Methods and applications in fluorescence · 2019Article
- Rhinella marina oocytes: a suitable alternative expression system for functional characterization of aquaglyceroporins.Scientific reports · 2019Article
- Mistargeting of a truncated Na-K-2Cl cotransporter in epithelial cells.American journal of physiology. Cell physiology · 2018Article
- Heterologous Protein Expression in theCold Spring Harbor protocols · 2018Article
- Plant Cation-Chloride Cotransporters (CCC): Evolutionary Origins and Functional Insights.International journal of molecular sciences · 2018Review
- A patient with multisystem dysfunction carries a truncation mutation in humanCold Spring Harbor molecular case studies · 2016Article
- Expressing and characterizing mechanosensitive channels in Xenopus oocytes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- A trafficking-deficient mutant of KCC3 reveals dominant-negative effects on K-Cl cotransport function.PloS one · 2013Article
- Calcium-binding protein 39 facilitates molecular interaction between Ste20p proline alanine-rich kinase and oxidative stress response 1 monomers.American journal of physiology. Cell physiology · 2012Article
- Kinetics of hyperosmotically stimulated Na-K-2Cl cotransporter in Xenopus laevis oocytes.American journal of physiology. Cell physiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To assess the effect of Xenopus husbandry on oocyte quality for membrane transport physiology experiments, we compared a recirculating-water housing system with a static-water system in a 23-mo study. Two groups of frogs (n = 8) were maintained separately for the entire study: one group was housed in a multiinvestigator centrally managed Xenopus facility, which consists of 33 tanks placed on a shared and recirculating water system; the other group was housed in a satellite facility used by a single investigator and consisting of static tanks placed in a dedicated cold-room. The activity of a heterologously expressed membrane transporter was assessed every 4 to 5 wk for a total of 23 mo. Activity of the mouse cotransporter NKCC1 was assessed through isotopic (86) Rb influx measurements under 2 experimental conditions: stimulation of cotransporter by coinjection of regulatory kinases and by exposure to a hypertonic solution. The results showed a significant difference in the level of ion fluxes under these 2 experimental conditions between the 2 groups of oocytes. During the entire period, oocytes isolated from frogs maintained in the static facility demonstrated consistently robust NKCC1 function, whereas oocytes isolated from frogs maintained in the recirculating facility showed inconsistent and weaker cotransporter function. Furthermore, the oocytes isolated from frogs maintained in the recirculating facility showed significant deterioration during the summer months (April to August), a seasonal variation that was muted in frog oocytes maintained in the static facility.
Indexed as
Identifiers
21333163PMC3035403W151712821What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.