ArticleExperimental biology and medicine (Maywood, N.J.)2011
Hierarchical architecture influences calcium dynamics in engineered cardiac muscle.
Article in Experimental biology and medicine (Maywood, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed.
- In vitro sex-specific function-structure relationship in neonatal rat cardiac monolayers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Tracking single hiPSC-derived cardiomyocyte contractile function using CONTRAX an efficient pipeline for traction force measurement.Nature communications · 2024Article
- Micropatterned fibrin scaffolds increase cardiomyocyte alignment and contractility for the fabrication of engineered myocardial tissue.Journal of biomedical materials research. Part A · 2023Article
- High-resolution spatiotemporal changes in dominant frequency and structural organization during persistent atrial fibrillation.PloS one · 2023Article
- Multicellular 3D Models for the Study of Cardiac Fibrosis.International journal of molecular sciences · 2022Review
- Cellular and Engineered Organoids for Cardiovascular Models.Circulation research · 2022Review
- Engineering aligned human cardiac muscle using developmentally inspired fibronectin micropatterns.Scientific reports · 2021Article
- Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering.Micromachines · 2021Review
- Mitochondrial architecture in cardiac myocytes depends on cell shape and matrix rigidity.Journal of molecular and cellular cardiology · 2021Article
- Regulation of calcium dynamics and propagation velocity by tissue microstructure in engineered strands of cardiac tissue.Integrative biology : quantitative biosciences from nano to macro · 2020Article
- Exploring cardiac form and function: A length-scale computational biology approach.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- Biomimetic cardiovascular platforms for in vitro disease modeling and therapeutic validation.Biomaterials · 2019Review
- Engineering cardiac microphysiological systems to model pathological extracellular matrix remodeling.American journal of physiology. Heart and circulatory physiology · 2018Review
- Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs.Scientific reports · 2018Article
- In silico study of multicellular automaticity of heterogeneous cardiac cell monolayers: Effects of automaticity strength and structural linear anisotropy.PLoS computational biology · 2018Article
- Toward improved myocardial maturity in an organ-on-chip platform with immature cardiac myocytes.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Maturation of human pluripotent stem cell derived cardiomyocytes is improved in cardiovascular construct.Cytotechnology · 2017Article
- In vitro models of the cardiac microenvironment to study myocyte and non-myocyte crosstalk: bioinspired approaches beyond the polystyrene dish.The Journal of physiology · 2017Review
- Recapitulation of microtissue models connected with real-time readout systems via 3D printing technology.Journal of thoracic disease · 2017Article
- Tissue Engineering Approaches in the Design of Healthy and PathologicalFrontiers in bioengineering and biotechnology · 2017Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Changes in myocyte cell shape and tissue structure are concurrent with changes in electromechanical function in both the developing and diseased heart. While the anisotropic architecture of cardiac tissue is known to influence the propagation of the action potential, the influence of tissue architecture and its potential role in regulating excitation-contraction coupling (ECC) are less well defined. We hypothesized that changes in the shape and the orientation of cardiac myocytes induced by spatial arrangement of the extracellular matrix (ECM) affects ECC. To test this hypothesis, we isolated and cultured neonatal rat ventricular cardiac myocytes on various micropatterns of fibronectin where they self-organized into tissues with varying degrees of anisotropy. We then measured the morphological features of these engineered myocardial tissues across several hierarchical dimensions by measuring cellular aspect ratio, myocyte area, nuclear density and the degree of cytoskeletal F-actin alignment. We found that when compared with isotropic tissues, anisotropic tissues have increased cellular aspect ratios, increased nuclear densities, decreased myocyte cell areas and smaller variances in actin alignment. To understand how tissue architecture influences cardiac function, we studied the role of anisotropy on intracellular calcium ([Ca(2+)](i)) dynamics by characterizing the [Ca(2+)](i)-frequency relationship of electrically paced tissues. When compared with isotropic tissues, anisotropic tissues displayed significant differences in [Ca(2+)](i) transients, decreased diastolic baseline [Ca(2+)](i) levels and greater [Ca(2+)](i) influx per cardiac cycle. These results suggest that ECM cues influence tissue structure at cellular and subcellular levels and regulate ECC.
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