ArticleDevelopment genes and evolution2011
Development of an RNA interference method in the cladoceran crustacean Daphnia magna.
Article in Development genes and evolution, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 109 citations in OpenAlex.
- Effects of RNAi-by-feeding on DNA methyltransferases inEnvironmental epigenetics · 2026Article
- HP1-Mediated Silencing of theJournal of developmental biology · 2025Article
- Sex-Biased Gene Expression of RNAi Pathway Components in the Sea Lice Caligus rogercresseyi.Marine biotechnology (New York, N.Y.) · 2025Article
- Baculovirus and Plasmid Vector-Mediated Gene Transfer inBio-protocol · 2025Article
- The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Heredity · 2025Article
- Maternal effects in the model system Daphnia: the ecological past meets the epigenetic future.Heredity · 2025Review
- Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna.Scientific reports · 2024Article
- Screening of morphology-related genes based on predator-induced transcriptome sequencing and the functional analysis ofCurrent zoology · 2024Article
- Identification of gene isoforms and their switching events between male and female embryos of the parthenogenetic crustacean Daphnia magna.Scientific reports · 2024Article
- Efficient CRISPR genome editing and integrative genomic analyses reveal the mosaicism of Cas-induced mutations and pleiotropic effects ofbioRxiv : the preprint server for biology · 2024Article
- Analysis of the genome-editing activity of microinjected CRISPR/Cas9 ribonucleoprotein complexes inmicroPublication biology · 2024Article
- RNAi-Based Therapy: Combating Shrimp Viral Diseases.Viruses · 2023Review
- Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis.Frontiers in zoology · 2023Article
- Review
- Development of transgenic Daphnia magna for visualizing homology-directed repair of DNA.Scientific reports · 2022Article
- CELF1 represses Doublesex1 expression via its 5' UTR in the crustacean Daphnia magna.PloS one · 2022Article
- Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide.Frontiers in zoology · 2021Review
- DNMT3.1 controls trade-offs between growth, reproduction, and life span under starved conditions in Daphnia magna.Scientific reports · 2021Article
- Production of genome-edited Daphnia for heavy metal detection by fluorescence.Scientific reports · 2020Article
- De Novo Transcriptome Assembly and Gene Expression Profiling of the CopepodMarine drugs · 2020Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Daphnids are small crustaceans ubiquitous in fresh water; they have been a subject of study in ecology, evolution, and environmental sciences for decades. To understand data accumulated in daphnid biology at the molecular level, expressed sequence tags and a genome sequence have been determined. However, these discoveries lead to the problem of how to understand the functions of newly discovered genes. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) is a useful tool to achieve specific gene silencing in nontransformable species. Hence, we established a technique to inject exogenous materials into ovulated eggs and developed a dsRNA-based RNAi method for Daphnia magna. Eggs were collected just after ovulation and injected with dsRNA specific to the Distal-less (Dll) gene, which functions in appendage development in invertebrates and vertebrates. We found that the dsRNA successfully triggered the degradation of Dll mRNAs, which induced the truncation of the second antenna in a dose-dependent manner. This effect was sequence specific in that: (1) an unrelated dsRNA did not induce any morphological abnormalities and (2) two non-overlapping Dll dsRNAs generated the same phenotype. This is the first report of an RNAi technique in D. magna and, together with the emerging genome sequences, will be useful for advancing knowledge of the molecular biology of daphnids.
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