ArticleNucleus (Austin, Tex.)
The nucleoporin Nup153 affects spindle checkpoint activity due to an association with Mad1.
Article in Nucleus (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Nucleoporins in Cancer: Functional Roles and Therapeutic Opportunities.Cancer discovery · 2026Article
- Cul3 substrate adaptor SPOP targets Nup153 for degradation.Molecular biology of the cell · 2025Article
- The nucleoporin Nup153 is the anchor for Kif1a during basal nuclear migration in brain progenitor cells.Cell reports · 2024Article
- ZC3HC1 is a structural element of the nuclear basket effecting interlinkage of TPR polypeptides.Molecular biology of the cell · 2022Article
- Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome.Cold Spring Harbor perspectives in biology · 2022Review
- Pathogenic variants in nucleoporin TPR (translocated promoter region, nuclear basket protein) cause severe intellectual disability in humans.Human molecular genetics · 2022Article
- Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex.RNA biology · 2022Article
- PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression.Scientific reports · 2021Article
- Mapping Proximity Associations of Core Spindle Assembly Checkpoint Proteins.Journal of proteome research · 2021Article
- Inhibition of Nuclear Pore Complex Formation Selectively Induces Cancer Cell Death.Cancer discovery · 2021Article
- Multifunctionality of F-rich nucleoporins.Biochemical Society transactions · 2020Review
- Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway.Nature communications · 2020Article
- Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplication.The Journal of cell biology · 2020Article
- NUP214 deficiency causes severe encephalopathy and microcephaly in humans.Human genetics · 2019Article
- The Arabidopsis nucleoporin NUP1 is essential for megasporogenesis and early stages of pollen development.Plant cell reports · 2019Article
- Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis.Cell division · 2019Article
- Nup133 Is Required for Proper Nuclear Pore Basket Assembly and Dynamics in Embryonic Stem Cells.Cell reports · 2018Article
- A Mad2-Mediated Translational Regulatory Mechanism Promoting S-Phase Cyclin Synthesis Controls Origin Firing and Survival to Replication Stress.Molecular cell · 2018Article
- Seh1 targets GATOR2 and Nup153 to mitotic chromosomes.Journal of cell science · 2018Article
- Nucleoporins redistribute inside the nucleus after cell cycle arrest induced by histone deacetylases inhibition.Nucleus (Austin, Tex.) · 2017Article
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleoporin Nup153 is known to play pivotal roles in nuclear import and export in interphase cells and as the cell transitions into mitosis, Nup153 is involved in nuclear envelope breakdown. In this study, we demonstrate that the interaction of Nup153 with the spindle assembly checkpoint protein Mad1 is important in the regulation of the spindle checkpoint. Overexpression of human Nup153 in HeLa cells leads to the appearance of multinucleated cells and induces the formation of multipolar spindles. Importantly, it causes inactivation of the spindle checkpoint due to hypophosphorylation of Mad1. Depletion of Nup153 using RNA interference results in the decline of Mad1 at nuclear pores during interphase and more significantly causes a delayed dissociation of Mad1 from kinetochores in metaphase and an increase in the number of unresolved midbodies. In the absence of Nup153 the spindle checkpoint remains active. In vitro studies indicate direct binding of Mad1 to the N-terminal domain of Nup153. Importantly, Nup153 binding to Mad1 affects Mad1's phosphorylation status, but not its ability to interact with Mad2. Our data suggest that Nup153 levels regulate the localization of Mad1 during the metaphase/anaphase transition thereby affecting its phoshorylation status and in turn spindle checkpoint activity and mitotic exit.
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