ArticleNucleus (Austin, Tex.)
An in vitro model for Pelger-Huët anomaly: stable knockdown of lamin B receptor in HL-60 cells.
Article in Nucleus (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Envelope-Limited Chromatin Sheets (ELCS) Formation in The Nuclear Envelope of HL-60/S4 Cells.bioRxiv : the preprint server for biology · 2026Article
- An integrated multi-omics and network analysis of neutrophil differentiation from initial- to late-stage.Genome biology · 2026Article
- Nuclear Pore Complexes in Various States of HL-60/S4 Cells.bioRxiv : the preprint server for biology · 2026Article
- Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation.Nature cell biology · 2025Article
- Confinement by Liquid-Liquid Interface Replicates In Vivo Neutrophil Deformations and Elicits Bleb-Based Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Investigating neutrophil responses to stimuli: Comparative analysis of reactive species-dependent and independent mechanisms.Redox biology · 2025Article
- Tubular Lamin- and Emerin-Lined Nuclear Envelope Invaginations Isolate Chromatin Domains for Gene Expression Regulation.Sub-cellular biochemistry · 2025Review
- Blended Phenotype of Pelger-Huet Anomaly with Osteochondroma and Autosomal Recessive Deafness with Enlarged Vestibular Aqueduct.Molecular syndromology · 2022Article
- The Nuclear Envelope as a Regulator of Immune Cell Function.Frontiers in immunology · 2022Review
- Interphase epichromatin: last refuge for the 30-nm chromatin fiber?Chromosoma · 2021Review
- Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells.Scientific reports · 2021Article
- Lamin A/C and the Immune System: One Intermediate Filament, Many Faces.International journal of molecular sciences · 2020Review
- Inhibition of PP2A activity by HExperimental & molecular medicine · 2019Article
- The Neutrophil Nucleus: An Important Influence on Neutrophil Migration and Function.Frontiers in immunology · 2018Review
- Review
- Nucleosome repositioning during differentiation of a human myeloid leukemia cell line.Nucleus (Austin, Tex.) · 2017Article
- Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells.Nucleus (Austin, Tex.) · 2017Article
- Cancer cell migration in 3D tissue: negotiating space by proteolysis and nuclear deformability.Cell adhesion & migration · 2015Article
- Sizing and shaping the nucleus: mechanisms and significance.Current opinion in cell biology · 2014Review
- ELCS in ice: cryo-electron microscopy of nuclear envelope-limited chromatin sheets.Chromosoma · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The principal human blood granulocyte (neutrophil) possesses a lobulated and deformable nucleus, important to facilitate rapid egress from blood vessels as these cells migrate to sites of bacterial or fungal infection. This unusual nuclear shape is a product of elevated levels of an integral membrane protein of the nuclear envelope lamin B receptor (LBR) and of decreased amounts of lamin A/C. In humans, a genetic deficiency of LBR produces Pelger-Huët anomaly, resulting in blood neutrophils that exhibit hypolobulated nuclei with redistributed heterochromatin. Structural changes in nuclear architecture occur during granulopoiesis within bone marrow. The exact mechanisms of this nuclear shape change and of heterochromatin redistribution remain largely unknown. As a tool to facilitate analysis of these mechanisms, a stable LBR knockdown subline of HL-60 cells was established. During in vitro granulopoiesis induced with retinoic acid, the LBR knockdown cells retain an ovoid shaped nucleus with reduced levels of lamin A/C; while, the parent cells develop highly lobulated nuclei. In contrast, macrophage forms induced in LBR knockdown cells by in vitro treatment with phorbol ester were indistinguishable from the parent cells, judged by both nuclear shape and attached cell morphology. The capability of differentiation of LBR knockdown HL-60 cells should facilitate a detailed analysis of the molecular relationship between LBR levels, granulocyte nuclear shape and heterochromatin distribution.
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