ArticleBioengineered bugs
A short story about a big magic bug.
Article in Bioengineered bugs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
- Bacillus megaterium favours CO₂ mineralization into CaCO₃ over the ureolytic pathway.Scientific reports · 2025Article
- Biofertilizer use in the United States: definition, regulation, and prospects.Applied microbiology and biotechnology · 2024Review
- The Superiority ofMicroorganisms · 2022Article
- Enzyme Profiling and Identification of Endophytic and Rhizospheric Bacteria Isolated fromMicroorganisms · 2022Article
- A Plant Endophytic BacteriumMicroorganisms · 2022Article
- Construction and Application of a Plasmid-Based Signal Peptide Library for Improved Secretion of Recombinant Proteins withMicroorganisms · 2022Article
- Combinatorial approach for improved production of whole-cell 3-aminopropionic acid in recombinant3 Biotech · 2021Article
- Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant Bacillus megaterium in fed-batch bioreactors.Bioprocess and biosystems engineering · 2021Article
- A rare case of Bacillus megaterium soft tissues infection.Acta bio-medica : Atenei Parmensis · 2020Review
- Phage G Structure at 6.1 Å Resolution, Condensed DNA, and Host Identity Revision to a Lysinibacillus.Journal of molecular biology · 2020Article
- Engineered microbial biofuel production and recovery under supercritical carbon dioxide.Nature communications · 2019Article
- Cell-gel interactions of in-gel propagating bacteria.BMC research notes · 2018Article
- A look into a multifunctional toolbox: endophytic Bacillus species provide broad and underexploited benefits for plants.World journal of microbiology & biotechnology · 2018Review
- Recapture probability, flight morphology, and microorganisms.Current zoology · 2018Article
- Isolation, Development, and Genomic Analysis ofFrontiers in microbiology · 2018Article
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
- Analysis of cultivable microbiota and diet intake pattern of the long-lived naked mole-rat.Gut pathogens · 2016Article
- Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity.Applied and environmental microbiology · 2015Article
- Complete Genome Sequence of Bacillus megaterium Siphophage Stills.Genome announcements · 2015Article
- Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium.Microbial cell factories · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacillus megaterium, the "big beast," is a Gram-positive bacterium with a size of 4 × 1.5 µm. During the last years, it became more and more popular in the field of biotechnology for its recombinant protein production capacity. For the purpose of intra- as well as extracellular protein synthesis several vectors were constructed and commercialized (MoBiTec GmbH, Germany). On the basis of two compatible vectors, a T7 RNA polymerase driven protein production system was established. Vectors for chromosomal integration enable the direct manipulation of the genome. The vitamin B(12) biosynthesis of B. megaterium served as a model for the systematic development of a production strain using these tools. For this purpose, the overexpression of chromosomal and plasmid encoded genes and operons, the synthesis of anti-sense RNA for gene silencing, the removal of inhibitory regulatory elements in combination with the utilization of strong promoters, directed protein design, and the recombinant production of B(12) binding proteins to overcome feedback inhibition were successfully employed. For further system biotechnology based optimization strategies the genome sequence will provide a closer look into genomic capacities of B. megaterium. DNA arrays are available. Proteome, fluxome and metabolome analyses are possible. All data can be integrated by using a novel bioinformatics platform. Finally, the size of the "big beast" B. megaterium invites for cell biology research projects. All these features provide a solid basis for challenging biotechnological approaches.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.