ArticleBioengineered bugs
Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants.
Article in Bioengineered bugs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- High-Level Production of a Recombinant Protein inInternational journal of molecular sciences · 2024Article
- TheHorticulture research · 2023Article
- Nep1-Like Proteins From the Biocontrol AgentFrontiers in plant science · 2022Article
- Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect.Cellular and molecular life sciences : CMLS · 2021Review
- Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies.BMC research notes · 2020Article
- Glutaredoxin GRXS17 Associates with the Cytosolic Iron-Sulfur Cluster Assembly Pathway.Plant physiology · 2016Article
- Expression of synthetic human tropoelastin (hTE) protein in Nicotiana tabacum.GM crops & food · 2015Article
- Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors.PloS one · 2013Article
- The use of plants for the production of therapeutic human peptides.Plant cell reports · 2012Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently described an efficient transient expression system mediated by Agrobacterium tumefaciens for the production of HIV-1 Nef protein in Nicotiana benthamiana plants. In order to enhance the yield of recombinant protein we assayed the effect of three gene-silencing viral suppressor proteins (P25 of Potato Virus X, P19 of Artichoke Mottled Crinckle virus and Tomato Bushy Stunt virus) on Nef expression levels. Results demonstrated that AMCV-P19 gave the highest Nef yield (1.3% of total soluble protein) and that this effect was correlated to a remarkable decrease of Nef-specific small interfering RNAs (siRNAs) indicating an effective modulation of RNA silencing mechanisms. Here we report additional data on the production of different heterologous proteins including human immunoglobulin heavy and light chains and a virus coat protein that demonstrate the robustness of this co-agroinfiltration expression system boosted by the AMCV-P19 gene-silencing suppressor.
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