ArticleBioengineered bugs
Homologous expression of aspartokinase (ask) gene in Streptomyces clavuligerus and its hom-deleted mutant: effects on cephamycin C production.
Article in Bioengineered bugs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 19 citations in OpenAlex.
- In-depth comparative transcriptome analysis of Purpureocillium sp. CB1 under cadmium stress.Applied microbiology and biotechnology · 2023Article
- AflQ1-Q2 represses lincomycin biosynthesis via multiple cascades in Streptomyces lincolnensis.Applied microbiology and biotechnology · 2023Article
- Transcriptomic and metabolomic analyses for providing insights into the influence of polylysine synthetase on the metabolism of Streptomyces albulus.Microbial cell factories · 2022Article
- An integrative-omics analysis of an industrial clavulanic acid-overproducing Streptomyces clavuligerus.Applied microbiology and biotechnology · 2022Article
- Optimization of the precursor supply for an enhanced FK506 production inFrontiers in bioengineering and biotechnology · 2022Article
- Homologous expression of lysA encoding diaminopimelic acid (DAP) decarboxylase reveals increased antibiotic production in Streptomyces clavuligerus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Transcriptome-Based Identification of a Strong Promoter for Hyper-production of Natamycin in Streptomyces.Current microbiology · 2019Article
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6 authors at 1 institution in 1 country.
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Abstract
In this study, the effect of homologous multiple copies of the ask gene, which encodes aspartokinase catalyzing the first step of the aspartate pathway, on cephamycin C biosynthesis in S. clavuligerus NRRL 3585 and its hom mutant was investigated. The intracellular pool levels of aspartate pathway amino acids accorded well with the Ask activity levels in TB3585 and AK39. When compared with the control strain carrying vector alone without any gene insert, amplification of the ask gene in the wild strain resulted in a maximum of 3.1- and 3.3-fold increase in specific, 1.7- and 1.9-fold increase in volumetric cephamycin C production when grown in trypticase soy broth (TSB) and a modified chemically defined medium (mCDM), respectively. However, expression of multicopy ask gene in a hom-deleted background significantly decreased cephamycin C yields when the cells were grown in either TSB or mCDM, most probably due to physiological disturbance resulting from enzyme overexpression and high copy number plasmid burden in an auxotrophic host, respectively.
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