ArticleBiomedical optics express2010
A cylindrical zoom lens unit for adjustable optical sectioning in light sheet microscopy.
Article in Biomedical optics express, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 20 citations in OpenAlex.
- Online Monitoring of the Concentrations of Amorphous and Crystalline Mesoscopic Species Present in Solution.Crystal growth & design · 2022Article
- Multi-photon attenuation-compensated light-sheet fluorescence microscopy.Scientific reports · 2020Article
- Fast imaging of live organisms with sculpted light sheets.Scientific reports · 2015Article
- A single molecule view on Dbp5 and mRNA at the nuclear pore.Nucleus (Austin, Tex.)Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light sheet microscopy became a powerful tool in life sciences. Often, however, the sheet geometry is fixed, whereas it would be advantageous to adjust the sheet geometry to specimens of different dimensions. Therefore we developed an afocal cylindrical zoom lens system comprising only 5 lenses and a total system length of less than 160 mm. Two movable optical elements were directly coupled, so that the zoom factor could be adjusted from 1x to 6.3x by a single motor. Using two different illumination objectives we achieved a light sheet thickness ranging from 2.4 µm to 36 µm corresponding to lateral fields of 54 µm to 12.3 mm, respectively. Polytene chromosomes of salivary gland cell nuclei of C.tentans larvae were imaged in vivo to demonstrate the advantages in image contrast by imaging with different light sheet dimensions.
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