ArticleMolecular therapy : the journal of the American Society of Gene Therapy2011
Gene editing of human embryonic stem cells via an engineered baculoviral vector carrying zinc-finger nucleases.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed.
- Review
- EXTL3 and NPC1 are mammalian host factors for Autographa californica multiple nucleopolyhedrovirus infection.Nature communications · 2024Article
- Development of a baculoviral CRISPR/Cas9 vector system for beta-2-microglobulin knockout in human pluripotent stem cells.Molecular genetics and genomics : MGG · 2024Article
- Insights of CRISPR-Cas systems in stem cells: progress in regenerative medicine.Molecular biology reports · 2022Review
- Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells.PloS one · 2017Article
- Site-Specific Genome Engineering in Human Pluripotent Stem Cells.International journal of molecular sciences · 2016Review
- Targeted, homology-driven gene insertion in stem cells by ZFN-loaded 'all-in-one' lentiviral vectors.eLife · 2016Article
- Dissecting diabetes/metabolic disease mechanisms using pluripotent stem cells and genome editing tools.Molecular metabolism · 2015Review
- Review
- Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery.Scientific reports · 2014Article
- Visualization of intracellular pathways of engineered baculovirus in mammalian cells.Virus research · 2014Article
- Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system.Molecular therapy. Methods & clinical development · 2014Article
- Emerging gene editing strategies for Duchenne muscular dystrophy targeting stem cells.Frontiers in physiology · 2014Review
- Zinc finger nuclease-expressing baculoviral vectors mediate targeted genome integration of reprogramming factor genes to facilitate the generation of human induced pluripotent stem cells.Stem cells translational medicine · 2013Article
- Newer gene editing technologies toward HIV gene therapy.Viruses · 2013Review
- Advances in homology directed genetic engineering of human pluripotent and adult stem cells.World journal of stem cells · 2013Article
- Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells.Nucleic acids research · 2013Article
- Systemic delivery of fusogenic membrane glycoprotein-expressing neural stem cells to selectively kill tumor cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Generation of an HIV resistant T-cell line by targeted "stacking" of restriction factors.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Baculovirus: an insect-derived vector for diverse gene transfer applications.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Human embryonic stem (hES) cells are renewable cell sources that have potential applications in regenerative medicine. The development of technologies to produce permanent and site-specific genome modifications is in demand to achieve future medical implementation of hES cells. We report herein that a baculoviral vector (BV) system carrying zinc-finger nucleases (ZFNs) can successfully modify the hES cell genome. BV-mediated transient expression of ZFNs specifically disrupted the CCR5 locus in transduced cells and the modified cells exhibited resistance to HIV-1 transduction. To convert the BV to a gene targeting vector, a DNA donor template and ZFNs were incorporated into the vector. These hybrid vectors yielded permanent site-specific gene addition in both immortalized human cell lines (10%) and hES cells (5%). Modified hES cells were both karyotypically normal and pluripotent. These results suggest that this baculoviral delivery system can be engineered for site-specific genetic manipulation in hES cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.