ArticlePlant physiology2011
High levels of bioplastic are produced in fertile transplastomic tobacco plants engineered with a synthetic operon for the production of polyhydroxybutyrate.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 114 citations in OpenAlex.
- Plastid Engineering for Photosynthesis-Driven Synthesis of Hyaluronic Acid in Tobacco.Plant biotechnology journal · 2026Article
- RNA elements and their biotechnological applications in plants.The New phytologist · 2025Review
- Artificial Biopolymers Derived from Transgenic Plants: Applications and Properties-A Review.International journal of molecular sciences · 2024Review
- Exploring recent progress of molecular farming for therapeutic and recombinant molecules in plant systems.Heliyon · 2024Review
- Chloroplast Engineering: Fundamental Insights and Its Application in Amelioration of Environmental Stress.Applied biochemistry and biotechnology · 2023Review
- Abatement of microfibre pollution and detoxification of textile dye - Indigo by engineered plant enzymes.Plant biotechnology journal · 2023Article
- Article
- Closing the Gap between Bio-Based and Petroleum-Based Plastic through Bioengineering.Microorganisms · 2022Review
- Genetic Containment for Molecular Farming.Plants (Basel, Switzerland) · 2022Review
- Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- 3-Hydroxybutyrate as a Metabolite and a Signal Molecule Regulating Processes of Living Organisms.Biomolecules · 2021Review
- Field evaluation of one Cu-resistant somaclonal variant and two clones of tobacco for copper phytoextraction at a wood preservation site.Environmental science and pollution research international · 2020Article
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Accumulation of high-value bioproductsProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Reconfiguring Plant Metabolism for Biodegradable Plastic Production.Biodesign research · 2020Review
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- Synthetic Botany.Cold Spring Harbor perspectives in biology · 2017Review
- A bifunctional aminoglycoside acetyltransferase/phosphotransferase conferring tobramycin resistance provides an efficient selectable marker for plastid transformation.Plant molecular biology · 2017Article
- Edible plants for oral delivery of biopharmaceuticals.British journal of clinical pharmacology · 2017Review
- Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants.Plant biotechnology (Tokyo, Japan) · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An optimized genetic construct for plastid transformation of tobacco (Nicotiana tabacum) for the production of the renewable, biodegradable plastic polyhydroxybutyrate (PHB) was designed using an operon extension strategy. Bacterial genes encoding the PHB pathway enzymes were selected for use in this construct based on their similarity to the codon usage and GC content of the tobacco plastome. Regulatory elements with limited homology to the host plastome yet known to yield high levels of plastidial recombinant protein production were used to enhance the expression of the transgenes. A partial transcriptional unit, containing genes of the PHB pathway and a selectable marker gene encoding spectinomycin resistance, was flanked at the 5' end by the host plant's psbA coding sequence and at the 3' end by the host plant's 3' psbA untranslated region. This design allowed insertion of the transgenes into the plastome as an extension of the psbA operon, rendering the addition of a promoter to drive the expression of the transgenes unnecessary. Transformation of the optimized construct into tobacco and subsequent spectinomycin selection of transgenic plants yielded T0 plants that were capable of producing up to 18.8% dry weight PHB in samples of leaf tissue. These plants were fertile and produced viable seed. T1 plants producing up to 17.3% dry weight PHB in samples of leaf tissue and 8.8% dry weight PHB in the total biomass of the plant were also isolated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.