ArticleJournal of clinical microbiology2011
Development and validation of a multiplex PCR for detection of Scedosporium spp. in respiratory tract specimens from patients with cystic fibrosis.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Diagnosis and management of invasive fungal infections due to non-Aspergillus moulds.The Journal of antimicrobial chemotherapy · 2025Review
- Scedosporiosis and lomentosporiosis: modern perspectives on these difficult-to-treat rare mold infections.Clinical microbiology reviews · 2024Review
- Non-Journal of fungi (Basel, Switzerland) · 2023Review
- Review
- Review
- Multi-Omics Approaches: The Key to Improving Respiratory Health in People With Cystic Fibrosis?Frontiers in pharmacology · 2020Review
- Comparative transcriptome analysis unveils the adaptative mechanisms ofComputational and structural biotechnology journal · 2020Article
- Challenges in Laboratory Detection of Fungal Pathogens in the Airways of Cystic Fibrosis Patients.Mycopathologia · 2018Article
- Spontaneous Remission of Subcutaneous Scedosporiosis Caused by Scedosporium dehoogii in a Psoriatic Patient.Mycopathologia · 2017Article
- Rapid development of a mycotic aneurysm of the intracranial artery secondary toMedical mycology case reports · 2016Article
- Purification and characterization of a mycelial catalase from Scedosporium boydii, a useful tool for specific antibody detection in patients with cystic fibrosis.Clinical and vaccine immunology : CVI · 2015Article
- Classical pathology of sympathetic ophthalmia presented in a unique case.The open ophthalmology journal · 2014Article
- Impact of multidrug-resistant organisms on patients considered for lung transplantation.Infectious disease clinics of North America · 2013Review
- State of the art diagnostic of mold diseases: a practical guide for clinicians.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Review
- Detection, identification, and distribution of fungi in bronchoalveolar lavage specimens by use of multilocus PCR coupled with electrospray ionization/mass spectrometry.Journal of clinical microbiology · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of Scedosporium infections in diverse groups of individuals, which are often treatment refractory, warrants timely and accurate laboratory diagnosis. Species- or group-specific primers based on internal transcribed spacer (ITS) sequence polymorphisms were designed for Scedosporium aurantiacum, Scedosporium dehoogii, Scedosporium prolificans, Pseudallescheria boydii species complex (former clade 5)/Pseudallescheria apiosperma (formerly classified as S. apiospermum sensu lato) and Pseudallescheria minutispora. Primers for S. aurantiacum, S. prolificans, and P. boydii species complex/P. apiosperma were incorporated into a multiplex PCR assay for the detection and identification of the three major clinically important Scedosporium species and validated using sputum specimens collected from patients seen at a major Australian cystic fibrosis clinic. The multiplex PCR assay showed 100% specificity in identifying the three major clinically relevant Scedosporium species from pure culture. When evaluated using DNA extracts from sputa, sensitivity and specificity of the multiplex PCR assay were 62.1% and 97.2%, respectively. This highly species-specific multiplex PCR assay offers a rapid and simple method of detection of the most clinically important Scedosporium species in respiratory tract specimens.
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