ArticleJournal of clinical microbiology2011
Development of a real-time PCR assay for detecting and quantifying human bocavirus 2.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Tracking the occurrence and fate of human bocavirus 2 in municipal wastewater from a small city.Molecular biology reports · 2026Article
- Human Bocavirus Circulating in Patients with Acute Gastroenteritis in Taiwan, 2018-2022.Viruses · 2024Article
- Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections.Pathogens (Basel, Switzerland) · 2020Article
- Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases.Journal of medical microbiology · 2020Article
- Whole-genome sequencing analysis of human bocavirus detected in South Korea.Epidemiology and infection · 2018Article
- Human Parvoviruses.Clinical microbiology reviews · 2017Review
- A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System.PloS one · 2016Article
- A two-tube multiplex reverse transcription PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes.BioMed research international · 2013Article
- Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions.Virology journal · 2012Article
- Human bocavirus types 1, 2 and 3 in acute gastroenteritis of childhood.Acta paediatrica (Oslo, Norway : 1992) · 2012Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Article
- Frequency of viral etiology in symptomatic adult upper respiratory tract infections.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human bocavirus 2 (HBoV2) is a parvovirus that has been recently identified in stool samples from children. Any association between the virus and clinical disease is unclear. A rapid, reliable diagnostic method is necessary to address this issue. In this study, we developed a sensitive and specific HBoV2 quantitative real-time PCR assay that targets the HBoV2 NP-1 gene, based on the TaqMan method. The assay could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the HBoV2 genome, with a dynamic range of 8 log units (10(1) to 10(8) copies). A clinical evaluation detected HBoV2 in 85 (24.6%) of 345 children with gastroenteritis, with viral loads ranging from 1.67 × 10(2) to 4.27 × 10(9) copies per ml of stool specimen.
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