ArticleJournal of the Royal Society, Interface2011
Nanogel surface coatings for improved single-molecule imaging substrates.
Article in Journal of the Royal Society, Interface, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- A theoretical analysis of single molecule protein sequencing via weak binding spectra.PloS one · 2019Article
- Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution.Communications biology · 2019Article
- Combined, Independent Small Molecule Release and Shape Memory via Nanogel-Coated Thiourethane Polymer Networks.Polymer chemistry · 2016Article
- A starting point for fluorescence-based single-molecule measurements in biomolecular research.Molecules (Basel, Switzerland) · 2014Review
- Ultralow protein adsorbing coatings from clickable PEG nanogel solutions: benefits of attachment under salt-induced phase separation conditions and comparison with PEG/albumin nanogel coatings.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Hydrophobic fluorescent probes introduce artifacts into single molecule tracking experiments due to non-specific binding.PloS one · 2013Article
- A systematic investigation of differential effects of cell culture substrates on the extent of artifacts in single-molecule tracking.PloS one · 2012Article
- Sensitive single-molecule protein quantification and protein complex detection in a microarray format.Proteomics · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Surfaces that resist protein adsorption are important for many bioanalytical applications. Bovine serum albumin (BSA) coatings and multi-arm poly(ethylene glycol) (PEG) coatings display low levels of non-specific protein adsorption and have enabled highly quantitative single-molecule (SM) protein studies. Recently, a method was developed for coating a glass with PEG-BSA nanogels, a promising hybrid of these two low-background coatings. We characterized the nanogel coating to determine its suitability for SM protein experiments. SM adsorption counting revealed that nanogel-coated surfaces exhibit lower protein adsorption than covalently coupled BSA surfaces and monolayers of multi-arm PEG, so this surface displays one of the lowest degrees of protein adsorption yet observed. Additionally, the nanogel coating was resistant to DNA adsorption, underscoring the utility of the coating across a variety of SM experiments. The nanogel coating was found to be compatible with surfactants, whereas the BSA coating was not. Finally, applying the coating to a real-world study, we found that single ligand molecules could be tethered to this surface and detected with high sensitivity and specificity by a digital immunoassay. These results suggest that PEG-BSA nanogel coatings will be highly useful for the SM analysis of proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.