ArticleMolecular microbiology2011
RatA (YfjG), an Escherichia coli toxin, inhibits 70S ribosome association to block translation initiation.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 69 citations in OpenAlex.
- A Microscale Platform for the Comprehensive Analysis of Bacterial Translation Initiation.International journal of molecular sciences · 2026Article
- Characterization of Pathogenic Photobacterium damselae subsp. damselae From Whiteleg Shrimp (Litopenaeus vannamei): An Emerging Cause of Mass Mortality in the Republic of Korea.Transboundary and emerging diseases · 2026Article
- Genomic characterization of Comamonas kerstersii isolated from diarrheal patients in Bangladesh.PloS one · 2026Article
- Lessons from RatA: Why the Basics in Molecular Biology Are Still Crucial!International journal of molecular sciences · 2025Article
- Article
- Chromosomal Type II Toxin-Antitoxin Systems May Enhance Bacterial Fitness of a Hybrid PathogenicToxins · 2024Article
- Applications of toxin-antitoxin systems in synthetic biology.Engineering microbiology · 2023Review
- Identification and Characterization of HEPN-MNT Type II TA System from Methanothermobacter thermautotrophicus ΔH.Journal of microbiology (Seoul, Korea) · 2023Article
- Toxin-antitoxin systems in bacterial pathogenesis.Heliyon · 2023Review
- Toxin-antitoxin systems and their medical applications: current status and future perspective.Applied microbiology and biotechnology · 2021Review
- Genome Mining Shows Ubiquitous Presence and Extensive Diversity of Toxin-Antitoxin Systems inFrontiers in microbiology · 2021Article
- PasT of Escherichia coli sustains antibiotic tolerance and aerobic respiration as a bacterial homolog of mitochondrial Coq10.MicrobiologyOpen · 2020Article
- Computational evidence for antitoxins associated with RelE/ParE, RatA, Fic, and AbiEii-family toxins in Wolbachia genomes.Molecular genetics and genomics : MGG · 2020Article
- Review
- Type II and type IV toxin-antitoxin systems show different evolutionary patterns in the global Klebsiella pneumoniae population.Nucleic acids research · 2020Article
- The role of major virulence factors and pathogenicity of adherent-invasivePrzeglad gastroenterologiczny · 2020Review
- Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function.Journal of lipid research · 2019Article
- SLING: a tool to search for linked genes in bacterial datasets.Nucleic acids research · 2018Article
- Disassembly of theProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Toxins of Prokaryotic Toxin-Antitoxin Systems with Sequence-Specific Endoribonuclease Activity.Toxins · 2017Review
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2 authors.
Funding
Abstract
RatA (YfjG) is a toxin encoded by the ratA-ratB (yfjG-yfjF) operon on the Escherichia coli genome. Induction of RatA led to the inhibition of protein synthesis, while DNA and RNA synthesis was not affected. The stability of mRNAs was also unchanged as judged by in vivo primer extension experiments and by Northern blotting analysis. The ribosome profile of the cells overexpressing RatA showed that 70S ribosomes as well as polysomes significantly decreased with concomitant increase of 50S and 30S subunits. The addition of purified RatA to a cell-free system inhibited the formation of 70S ribosomes even in the presence of 6 mM Mg(2+) . RatA was specifically associated with 50S subunits, indicating that it binds to 50S subunits to block its association with 30S subunits leading to the inhibition of formation of 70S ribosomes. However, RatA did not cause dissociation of 70S ribosomes and its anti-association activity was blocked by paromomycin, an inhibitor for IF3, an essential initiation factor, having 21% sequence homology with RatA. Here we demonstrate that RatA is a new E. coli toxin, which effectively blocks the translation initiation step. We propose that this toxin of previously unknown function be renamed as RatA (Ribosome association toxin A).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.