ArticleAnalytical chemistry2011
Analysis of biogenic amines in a single Drosophila larva brain by capillary electrophoresis with fast-scan cyclic voltammetry detection.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Capillary Electrophoresis With Amperometric Detection for Neurotransmitter Analysis: Principles, Electrode Materials, Methodologies, and Applications.Electrophoresis · 2026Review
- Machine Learning for Separating Dopamine and Octopamine Electrochemical Signals in Drosophila.Analytical chemistry · 2026Article
- An octopamine-specific GRAB sensor reveals a monoamine relay circuitry that boosts aversive learning.National science review · 2024Article
- An octopamine-specific GRAB sensor reveals a monoamine relay circuitry that boosts aversive learning.bioRxiv : the preprint server for biology · 2024Article
- Ring Finger Protein 11 (RNF11) Modulates Dopamine Release in Drosophila.Neuroscience · 2021Article
- Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function.Addiction biology · 2020Article
- Recent advances in fast-scan cyclic voltammetry.The Analyst · 2020Review
- Evaluation of dual electrode configurations for microchip electrophoresis used for voltammetric characterization of electroactive species.The Analyst · 2020Article
- New optical methods for detecting monoamine neuromodulators.Current opinion in biomedical engineering · 2019Article
- Drosophila as a Model System for Neurotransmitter Measurements.ACS chemical neuroscience · 2018Review
- Determination of Biogenic Amines in Seawater Using Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection.Molecules (Basel, Switzerland) · 2018Article
- Nicotinic acetylcholine receptor (nAChR) mediated dopamine release in larval Drosophila melanogaster.Neurochemistry international · 2018Article
- Fast-Scan Cyclic Voltammetry (FSCV) Detection of Endogenous Octopamine in Drosophila melanogaster Ventral Nerve Cord.ACS chemical neuroscience · 2016Article
- Quantitative analysis of serotonin secreted by human embryonic stem cells-derived serotonergic neurons via pH-mediated online stacking-CE-ESI-MRM.Electrophoresis · 2016Article
- Quantification of Histamine and Carcinine in Drosophila melanogaster Tissues.ACS chemical neuroscience · 2016Article
- Microchip electrophoresis with electrochemical detection for the determination of analytes in the dopamine metabolic pathway.Electrophoresis · 2015Article
- RECENT DEVELOPMENTS IN ELECTROCHEMICAL SENSORS FOR THE DETECTION OF NEUROTRANSMITTERS FOR APPLICATIONS IN BIOMEDICINE.Analytical letters · 2015Article
- Analysis of neurotransmitter tissue content of Drosophila melanogaster in different life stages.ACS chemical neuroscience · 2015Article
- Microelectronics-based biosensors dedicated to the detection of neurotransmitters: a review.Sensors (Basel, Switzerland) · 2014Review
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila, the fruit fly, is a common model organism in biology; however, quantifying neurotransmitters in Drosophila is challenging because of the small size of the central nervous system (CNS). Here, we develop neurotransmitter quantification by capillary electrophoresis with fast-scan cyclic voltammetry (CE-FSCV) detection, which allows peak identification by both migration time and the cyclic voltammogram, in contrast to traditional amperometric detection which provides no chemical identification. Tissue content of biogenic amine neurotransmitters was determined in a single CNS dissected from a Drosophila larva. Low detection limits, 1 nM for dopamine and serotonin, 2.5 nM for tyramine, and 4 nM for octopamine, were achieved using field-amplified sample stacking by diluting the homogenized tissue with percholoric acid and acetonitrile. Two different strains of wild-type flies, Oregon R and Canton S, have similar dopamine and serotonin levels but different octopamine content. When flies are fed NSD-1015, which inhibits dopamine decarboxylase (Ddc) a synthesis enzyme in the dopamine and serotonin pathways, dopamine significantly decreases by 52%. A genetically altered driver line, Ddc-GAL4, had lower serotonin and dopamine content as did w(118) flies. When the Ddc-GAL4 line was used to produce flies overexpressing the serotonin synthesis enzyme tryptophan hydroxylase (Ddc-GAL4;UAS-Trh), the serotonin tissue content was greater than for Ddc-GAL4 but not significantly different than the wild-type. These results show that CE-FSCV is useful for monitoring the impact of genetic and pharmacological manipulations on the content of multiple neurotransmitters in a CNS from a Drosophila larva.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.