ArticlePlant physiology2011
Phenotypic and genomic analyses of a fast neutron mutant population resource in soybean.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 205 citations in OpenAlex.
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- Variation in shoot architecture traits and their relationship to canopy coverage and light interception in soybean (Glycine max).BMC plant biology · 2024Article
- Identification and characterization of a temperature sensitive chlorotic soybean mutant.bioRxiv : the preprint server for biology · 2024Article
- Rice mutants, selected under severe drought stress, show reduced stomatal density and improved water use efficiency under restricted water conditions.Frontiers in plant science · 2024Article
- Critical role for uricase and xanthine dehydrogenase in soybean nitrogen fixation and nodule development.The plant genome · 2023Article
- A chromosome 16 deletion conferring a high sucrose phenotype in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genetic mapping and functional genomics of soybean seed protein.Molecular breeding : new strategies in plant improvement · 2023Article
- Identification and Characterization of Common Bean (Plants (Basel, Switzerland) · 2023Article
- Review
- Biological and genomic analysis of a symbiotic nitrogen fixation defective mutant inFrontiers in plant science · 2023Article
- Genes Associated with Biological Nitrogen Fixation Efficiency Identified Using RNA Sequencing in Red Clover (Life (Basel, Switzerland) · 2022Article
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- Fast neutron mutagenesis in soybean enriches for small indels and creates frameshift mutations.G3 (Bethesda, Md.) · 2022Article
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- Geographic distribution of the E1 family of genes and their effects on reproductive timing in soybean.BMC plant biology · 2021Article
- Rapid identification of mutations caused by fast neutron bombardment in Medicago truncatula.Plant methods · 2021Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutagenized populations have become indispensable resources for introducing variation and studying gene function in plant genomics research. In this study, fast neutron (FN) radiation was used to induce deletion mutations in the soybean (Glycine max) genome. Approximately 120,000 soybean seeds were exposed to FN radiation doses of up to 32 Gray units to develop over 23,000 independent M2 lines. Here, we demonstrate the utility of this population for phenotypic screening and associated genomic characterization of striking and agronomically important traits. Plant variation was cataloged for seed composition, maturity, morphology, pigmentation, and nodulation traits. Mutants that showed significant increases or decreases in seed protein and oil content across multiple generations and environments were identified. The application of comparative genomic hybridization (CGH) to lesion-induced mutants for deletion mapping was validated on a midoleate x-ray mutant, M23, with a known FAD2-1A (for fatty acid desaturase) gene deletion. Using CGH, a subset of mutants was characterized, revealing deletion regions and candidate genes associated with phenotypes of interest. Exome resequencing and sequencing of PCR products confirmed FN-induced deletions detected by CGH. Beyond characterization of soybean FN mutants, this study demonstrates the utility of CGH, exome sequence capture, and next-generation sequencing approaches for analyses of mutant plant genomes. We present this FN mutant soybean population as a valuable public resource for future genetic screens and functional genomics research.
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