ArticleProceedings of the National Academy of Sciences of the United States of America2011
Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 83 citations in OpenAlex.
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- Ultrasensitive and Multiplexed Protein Imaging with Clickable and Cleavable Fluorophores.Analytical chemistry · 2024Article
- Synthesis of 5-(Aryl)amino-1,2,3-triazole-containing 2,1,3-Benzothiadiazoles via Azide-Nitrile Cycloaddition Followed by Buchwald-Hartwig Reaction.Molecules (Basel, Switzerland) · 2024Article
- Ultrasensitive and multiplexed protein imaging with clickable and cleavable fluorophores.bioRxiv : the preprint server for biology · 2023Article
- A nanopore interface for higher bandwidth DNA computing.Nature communications · 2022Article
- Multiplexed Single-Cell in Situ Protein Profiling.ACS measurement science au · 2022Review
- Sensitive Immunofluorescent Detection of the PRAME Antigen Using a Practical Antibody Conjugation Approach.International journal of molecular sciences · 2021Article
- Ultrasensitive and Multiplexed Protein Imaging with Cleavable Fluorescent Tyramide and Antibody Stripping.International journal of molecular sciences · 2021Article
- Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide.Cells · 2021Article
- Diastereoselective Synthesis of Aryl C-Glycosides from Glycosyl Esters via C-O Bond Homolysis.Angewandte Chemie (International ed. in English) · 2021Article
- Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide.Molecules (Basel, Switzerland) · 2021Article
- Highly Multiplexed Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization.Molecules (Basel, Switzerland) · 2020Article
- Highly Sensitive and Multiplexed In-Situ Protein Profiling with Cleavable Fluorescent Streptavidin.Cells · 2020Article
- Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity.Frontiers in cell and developmental biology · 2020Article
- A Lucifer-Based Environment-Sensitive Fluorescent PNA Probe for Imaging Poly(A) RNAs.Chembiochem : a European journal of chemical biology · 2018Article
- Single-CellFrontiers in cell and developmental biology · 2018Article
- BODIPY-Bacteriochlorin Energy Transfer Arrays: Toward Near-IR Emitters with Broadly Tunable, Multiple Absorption Bands.The Journal of organic chemistry · 2017Article
- Luminescent Carbon Dot Mimics Assembled on DNA.Journal of the American Chemical Society · 2017Article
- Efficient synthesis of fluorescent alkynyl C-nucleosides via Sonogashira coupling for the preparation of DNA-based polyfluorophores.Organic & biomolecular chemistry · 2016Article
- Assembling programmable FRET-based photonic networks using designer DNA scaffolds.Nature communications · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Most current approaches to multiantigen fluorescent imaging require overlaying of multiple images taken with separate filter sets as a result of differing dye excitation requirements. This requirement for false-color composite imaging prevents the user from visualizing multiple species in real time and disallows imaging of rapidly moving specimens. To address this limitation, here we investigate the use of oligodeoxyfluoroside (ODF) fluorophores as labels for antibodies. ODFs are short DNA-like oligomers with fluorophores replacing the DNA bases and can be assembled in many colors with excitation at a single wavelength. A DNA synthesizer was used to construct several short ODFs carrying a terminal alkyne group and having emission maxima of 410-670 nm. We developed a new approach to antibody conjugation, using Huisgen-Sharpless cycloaddition, which was used to react the alkynes on ODFs with azide groups added to secondary antibodies. Multiple ODF-tagged secondary antibodies were then used to mark primary antibodies. The set of antibodies was tested for spectral characteristics in labeling tubulin in HeLa cells and revealed a wide spectrum of colors, ranging from violet-blue to red with excitation through a single filter (340-380 nm). Selected sets of the differently labeled secondary antibodies were then used to simultaneously mark four antigens in fixed cells, using a single image and filter set. We also imaged different surface tumor markers on two live cell lines. Experiments showed that all colors could be visualized simultaneously by eye under the microscope, yielding multicolor images of multiple cellular antigens in real time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.