ArticleProceedings of the National Academy of Sciences of the United States of America2011
Membrane-inlet mass spectrometry reveals a high driving force for oxygen production by photosystem II.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Perspective on Nano-Enabled Photostimulation Biointerfaces.ACS nano · 2025Review
- Measurements of Oxygen Evolution in Photosynthesis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Solar energy conversion by photosystem II: principles and structures.Photosynthesis research · 2023Review
- NIR Luminescent Oxygen-Sensing Nanoparticles for Continuous Glucose and Lactate Monitoring.Biosensors · 2023Article
- Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II.Chemical science · 2022Article
- Water oxidation by photosystem II is the primary source of electrons for sustained HProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research.Frontiers in plant science · 2020Article
- Studying the oxidation of water to molecular oxygen in photosynthetic and artificial systems by time-resolved membrane-inlet mass spectrometry.Frontiers in plant science · 2013Review
- Ecophysiology of photosynthesis in macroalgae.Photosynthesis research · 2012Review
- Room temperature femtosecond X-ray diffraction of photosystem II microcrystals.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Misses during water oxidation in photosystem II are S state-dependent.The Journal of biological chemistry · 2012Article
- Algal evolution in relation to atmospheric CO2: carboxylases, carbon-concentrating mechanisms and carbon oxidation cycles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Thermodynamic limitations of photosynthetic water oxidation at high proton concentrations.The Journal of biological chemistry · 2011Article
- Adventures with cyanobacteria: a personal perspective.Frontiers in plant science · 2011Article
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5 authors.
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Abstract
Oxygenic photosynthesis is the basis for aerobic life on earth. The catalytic Mn(4)O(x)CaY(Z) center of photosystem II (PSII), after fourfold oxidation, extracts four electrons from two water molecules to yield dioxygen. This reaction cascade has appeared as a single four-electron transfer that occurs in typically 1 ms. Inevitable redox intermediates have so far escaped detection, probably because of very short lifetime. Previous attempts to stabilize intermediates by high O(2)-back pressure have revealed controversial results. Here we monitored by membrane-inlet mass spectrometry (MIMS) the production of from (18)O-labeled water against a high background of in a suspension of PSII-core complexes. We found neither an inhibition nor an altered pattern of O(2) production by up to 50-fold increased concentration of dissolved O(2). Lack of inhibition is in line with results from previous X-ray absorption and visible-fluorescence experiments, but contradictory to the interpretation of previous UV-absorption data. Because we used essentially identical experimental conditions in MIMS as had been used in the UV work, the contradiction was serious, and we found it was not to be resolved by assuming a significant slowdown of the O(2) release kinetics or a subsequent slow conformational relaxation. This calls for reevaluation of the less direct UV experiments. The direct detection of O(2) release by MIMS shows unequivocally that O(2) release in PSII is highly exothermic. Under the likely assumption that one H(+) is released in the S(4) → S(0) transition, the driving force at pH 6.5 and atmospheric O(2) pressure is at least 220 meV, otherwise 160 meV.
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