ArticleNucleic acids research2011
An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Mitochondrial genome annotation with MFannot: a critical analysis of gene identification and gene model prediction.Frontiers in plant science · 2023Article
- Mitochondrial Aminoacyl-tRNA Synthetase and Disease: The Yeast Contribution for Functional Analysis of Novel Variants.International journal of molecular sciences · 2021Review
- Naturally Occurring tRNAs With Non-canonical Structures.Frontiers in microbiology · 2020Review
- Naturally occurring dual recognition of tRNARNA biology · 2019Article
- Rapid Genetic Code Evolution in Green Algal Mitochondrial Genomes.Molecular biology and evolution · 2019Article
- How tRNAs dictate nuclear codon reassignments: Only a few can capture non-cognate codons.RNA biology · 2017Review
- Identification of distinct biological functions for four 3'-5' RNA polymerases.Nucleic acids research · 2016Article
- A novel nuclear genetic code alteration in yeasts and the evolution of codon reassignment in eukaryotes.Genome research · 2016Article
- The crystal structure of yeast mitochondrial ThrRS in complex with the canonical threonine tRNA.Nucleic acids research · 2016Article
- A Comparative Analysis of Mitochondrial Genomes in Eustigmatophyte Algae.Genome biology and evolution · 2016Article
- Non-Standard Genetic Codes Define New Concepts for Protein Engineering.Life (Basel, Switzerland) · 2015Review
- Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology.Nature reviews. Microbiology · 2015Review
- Towards a comprehensive picture of alloacceptor tRNA remolding in metazoan mitochondrial genomes.Nucleic acids research · 2015Article
- Pathways of Genetic Code Evolution in Ancient and Modern Organisms.Journal of molecular evolution · 2015Review
- Life without post-transcriptional addition of G-1: two alternatives for tRNAHis identity in Eukarya.RNA (New York, N.Y.) · 2015Article
- A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading.Nucleic acids research · 2014Article
- VARS2 and TARS2 mutations in patients with mitochondrial encephalomyopathies.Human mutation · 2014Article
- Massive programmed translational jumping in mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Natural reassignment of CUU and CUA sense codons to alanine in Ashbya mitochondria.Nucleic acids research · 2014Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The standard genetic code is used by most living organisms, yet deviations have been observed in many genomes, suggesting that the genetic code has been evolving. In certain yeast mitochondria, CUN codons are reassigned from leucine to threonine, which requires an unusual tRNA(Thr) with an enlarged 8-nt anticodon loop ( ). To trace its evolutionary origin we performed a comprehensive phylogenetic analysis which revealed that evolved from yeast mitochondrial tRNA(His). To understand this tRNA identity change, we performed mutational and biochemical experiments. We show that Saccharomyces cerevisiae mitochondrial threonyl-tRNA synthetase (MST1) could attach threonine to both and the regular , but not to the wild-type tRNA(His). A loss of the first nucleotide (G(-1)) in tRNA(His) converts it to a substrate for MST1 with a K(m) value (0.7 μM) comparable to that of (0.3 μM), and addition of G(-1) to allows efficient histidylation by histidyl-tRNA synthetase. We also show that MST1 from Candida albicans, a yeast in which CUN codons remain assigned to leucine, could not threonylate , suggesting that MST1 has coevolved with . Our work provides the first clear example of a recent recoding event caused by alloacceptor tRNA gene recruitment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.