ArticleBiophysical journal2011
Adhesion and merging of lipid bilayers: a method for measuring the free energy of adhesion and hemifusion.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Biophysical Considerations in Cell Fusion.Advances in experimental medicine and biology · 2026Review
- Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis.International journal of molecular sciences · 2023Article
- Osmotic Pressure Enables High-Yield Assembly of Giant Vesicles in Solutions of Physiological Ionic Strengths.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism.Biomolecules · 2020Article
- Asymmetric Bilayers by Hemifusion: Method and Leaflet Behaviors.Biophysical journal · 2019Article
- Supramolecular zippers elicit interbilayer adhesion of membranes producing cell death.Biochimica et biophysica acta. General subjects · 2018Article
- A stochastic model of active zone material mediated synaptic vesicle docking and priming at resting active zones.Scientific reports · 2017Article
- Mode of Action of Antimicrobial Peptides on E. coli Spheroplasts.Biophysical journal · 2016Article
- Membrane-mediated amyloid formation of PrP 106-126: A kinetic study.Biochimica et biophysica acta · 2015Article
- Physical properties of Escherichia coli spheroplast membranes.Biophysical journal · 2014Article
- Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore.Biomicrofluidics · 2013Article
- A novel phase of compressed bilayers that models the prestalk transition state of membrane fusion.Biophysical journal · 2012Article
- Determination of the strength of adhesion between lipid vesicles.TheScientificWorldJournal · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Lipid bilayers can be induced to adhere to each other by molecular mediators, and, depending on the lipid composition, such adhesion can lead to merging of the contacting monolayers in a process known as hemifusion. Such bilayer-bilayer reactions have never been systematically studied. In the course of our studies of membrane-active molecules, we encountered such reactions. We believe that they need to be understood whenever bilayer-bilayer interactions take place, such as during membrane fusion. For illustration, we discuss three examples: spontaneous adhesion between phospholipid bilayers induced by low pH, polymer-induced osmotic depletion attraction between lipid bilayers, and anionic lipid bilayers cross-bridged by multicationic peptides. Our purpose here is to describe a general method for studying such interactions. We used giant unilamellar vesicles, each of which was aspirated in a micropipette so that we could monitor the tension of the membrane and the membrane area changes during the bilayer-bilayer interaction. We devised a general method for measuring the free energy of adhesion or hemifusion. The results show that the energies of adhesion or hemifusion of lipid bilayers could vary over 2 orders of magnitude from -1 to -50 × 10(-5) J/m(2) in these examples alone. Our method can be used to measure the energy of transition in each step of lipid transformation during membrane fusion. This is relevant for current research on membrane fusion, which focuses on how fusion proteins induce lipid transformations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.