ArticleBiophysical journal2011
Three distinct modes of exocytosis revealed by amperometry in neuroendocrine cells.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Models of Intracellular Transport: Contradictions and Current Understanding.Sub-cellular biochemistry · 2026Review
- Fusion Pores as Regulators of Quantal Size and Cellular Physiology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- High-speed imaging reveals the bimodal nature of dense core vesicle exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A two-subpopulation model that reflects heterogeneity of large dense core vesicles in exocytosis.Cell cycle (Georgetown, Tex.) · 2022Article
- Nanoscale Amperometry Reveals that Only a Fraction of Vesicular Serotonin Content is Released During Exocytosis from Beta Cells.Angewandte Chemie (International ed. in English) · 2021Article
- Quantifying neurotransmitter secretion at single-vesicle resolution using high-density complementary metal-oxide-semiconductor electrode array.Nature communications · 2021Article
- Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes.Pflugers Archiv : European journal of physiology · 2021Review
- Antimalarial drugs impact chemical messenger secretion by blood platelets.Biochemistry and biophysics reports · 2020Article
- Spinal cord injury alters purinergic neurotransmission to mesenteric arteries in rats.American journal of physiology. Heart and circulatory physiology · 2020Article
- Chromaffin Cells of the Adrenal Medulla: Physiology, Pharmacology, and Disease.Comprehensive Physiology · 2019Article
- Estimating amperometric spike parameters resulting from quantal exocytosis using curve fitting seeded by a matched-filter algorithm.Journal of neuroscience methods · 2019Article
- Electrodeposited Gold on Carbon-Fiber Microelectrodes for Enhancing Amperometric Detection of Dopamine Release from Pheochromocytoma Cells.Analytical chemistry · 2018Article
- A matched-filter algorithm to detect amperometric spikes resulting from quantal secretion.Journal of neuroscience methods · 2018Article
- Amperometry methods for monitoring vesicular quantal size and regulation of exocytosis release.Pflugers Archiv : European journal of physiology · 2018Review
- Electrochemical measurement of quantal exocytosis using microchips.Pflugers Archiv : European journal of physiology · 2018Review
- Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods.Analytical chemistry · 2017Article
- Surface Trafficking of APP and BACE in Live Cells.Traffic (Copenhagen, Denmark) · 2015Article
- PC12 cells that lack synaptotagmin I exhibit loss of a subpool of small dense core vesicles.Biophysical journal · 2014Article
- Golgi's way: a long path toward the new paradigm of the intra-Golgi transport.Histochemistry and cell biology · 2013Review
- Amperometric measurements at cells support a role for dynamin in the dilation of the fusion pore during exocytosis.Chemphyschem : a European journal of chemical physics and physical chemistry · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurotransmission requires Ca(2+)-dependent release of secretory products through fusion pores that open and reclose (partial membrane distention) or open irreversibly (complete membrane distention). It has been challenging to distinguish between these release modes; however, in the work presented here, we were able to deduce different modes of depolarization-evoked exocytosis in neuroendocrine chromaffin and PC12 cells solely by analyzing amperometric recordings. After we determined the quantal size (Q), event half-width (t(50)), event amplitude (I(peak)), and event decay time constant (τ(decay)), we fitted scatter plots of log-transformed data with a mixture of one- and two-dimensional Gaussian distributions. Our analysis revealed three distinct and differently shaped clusters of secretory events, likely corresponding to different modes of exocytosis. Complete membrane distention, through fusion pores of widely varying conductances, accounted for 70% of the total amount of released catecholamine. Two different kinds of partial membrane distention (kiss-and-run and kiss-and-stay exocytosis), characterized by mode-specific fusion pores with unitary conductances, accounted for 20% and 10%, respectively. These results show that our novel one- and two-dimensional analysis of amperometric data reveals new release properties and enables one to distinguish at least three different modes of exocytosis solely by analyzing amperometric recordings.
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