ArticleBiophysical journal2011
Single ryanodine receptor channel basis of caffeine's action on Ca2+ sparks.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 71 citations in OpenAlex.
- Cardiotoxic Effects of Acute 4.5 mM Caffeine Exposure in 2-to-3-Day-Old Zebrafish are Attenuated by Tricaine.microPublication biology · 2026Article
- Toxicology of caffeine poisoning: molecular mechanisms, toxicokinetic profiles, and clinical implications.Frontiers in toxicology · 2026Review
- Introduction of a single carboxylic acid converts the cyclic oligomeric depsipeptide ent-verticilide from a ryanodine receptor 2 (RyR2) inhibitor to RyR2 activator.Molecular pharmacology · 2025Article
- Targeting ryanodine receptors with allopurinol and xanthine derivatives for the treatment of cardiac and musculoskeletal weakness disorders.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Role of ryanodine receptor cooperativity in CaThe Journal of physiology · 2024Article
- Isolated Cardiac Ryanodine Receptor Function Varies Between Mammals.The Journal of membrane biology · 2024Article
- Lidocaine induces apoptosis in head and neck squamous cell carcinoma through activation of bitter taste receptor T2R14.Cell reports · 2023Article
- Allosteric modulation of ryanodine receptor RyR1 by nucleotide derivatives.Structure (London, England : 1993) · 2023Article
- Treatment of Lethal Caffeine Overdose with Haemodialysis: A Case Report and Review.Journal of critical care medicine (Universitatea de Medicina si Farmacie din Targu-Mures) · 2022Article
- How does flecainide impact RyR2 channel function?The Journal of general physiology · 2022Article
- Adenosine ABritish journal of pharmacology · 2022Review
- The Cardiac Ryanodine Receptor Provides a Suitable Pathway for the Rapid Transport of Zinc (ZnCells · 2022Article
- The spatio-temporal properties of calcium transients in hippocampal pyramidal neuronsFrontiers in cellular neuroscience · 2022Article
- Caffeine: cardiorespiratory effects and tissue protection in animal models.Experimental animals · 2021Review
- A Type 2 Ryanodine Receptor Variant in the Helical Domain 2 Associated with an Impairment of the Adrenergic Response.Journal of personalized medicine · 2021Article
- The discovery and development of IP3 receptor modulators: an update.Expert opinion on drug discovery · 2021Article
- Endoplasmic Reticulum-Based Calcium Dysfunctions in Synucleinopathies.Frontiers in neurology · 2021Review
- Altered calcium handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts.JCI insight · 2020Article
- IP3R-Mediated Compensatory Mechanism for Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Cardiac Ryanodine Receptor Deficiency.Frontiers in cell and developmental biology · 2020Article
- Caffeine and Clinical Outcomes in Premature Neonates.Children (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Caffeine (1, 3, 7-trimethylxanthine) is a widely used pharmacological agonist of the cardiac ryanodine receptor (RyR2) Ca(2+) release channel. It is also a well-known stimulant that can produce adverse side effects, including arrhythmias. Here, the action of caffeine on single RyR2 channels in bilayers and Ca(2+) sparks in permeabilized ventricular cardiomyocytes is defined. Single RyR2 caffeine activation depended on the free Ca(2+) level on both sides of the channel. Cytosolic Ca(2+) enhanced RyR2 caffeine affinity, whereas luminal Ca(2+) essentially scaled maximal caffeine activation. Caffeine activated single RyR2 channels in diastolic quasi-cell-like solutions (cytosolic MgATP, pCa 7) with an EC(50) of 9.0 ± 0.4 mM. Low-dose caffeine (0.15 mM) increased Ca(2+) spark frequency ∼75% and single RyR2 opening frequency ∼150%. This implies that not all spontaneous RyR2 openings during diastole are associated with Ca(2+) sparks. Assuming that only the longest openings evoke sparks, our data suggest that a spark may result only when a spontaneous single RyR2 opening lasts >6 ms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.