ArticleMobile DNA2011
Characterization of a synthetic human LINE-1 retrotransposon ORFeus-Hs.
Article in Mobile DNA, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 69 citations in OpenAlex.
- The interferon-stimulated gene product HERC5 inhibits human LINE-1 retrotransposition with an ISGylation-independent mechanism.Nucleic acids research · 2026Article
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- Structural and Evolutionary Analysis ofInternational journal of molecular sciences · 2025Article
- LINE-1 ribonucleoprotein condensates bind DNA to enable nuclear entry during mitosis.Science advances · 2025Article
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- Transposons Acting as Competitive Endogenous RNAs: In-Silico Evidence from Datasets Characterised by L1 Overexpression.Biomedicines · 2022Article
- Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites.Nature communications · 2022Article
- Analysis of epigenetic features characteristic of L1 loci expressed in human cells.Nucleic acids research · 2022Article
- A Variety of Nucleic Acid Species Are Sensed by cGAS, Implications for Its Diverse Functions.Frontiers in immunology · 2022Review
- L1 retrotransposons exploit RNA mNature communications · 2021Article
- Cytoplasmic synthesis of endogenousProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Human L1 Transposition Dynamics Unraveled with Functional Data Analysis.Molecular biology and evolution · 2020Article
- Long interspersed element-1 ribonucleoprotein particles protect telomeric ends in alternative lengthening of telomeres dependent cells.Neoplasia (New York, N.Y.) · 2020Article
- Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication.Nature structural & molecular biology · 2020Article
- Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactions by Differential Affinity Immobilization.Bio-protocol · 2019Article
- LINE1 Derepression in Aged Wild-Type and SIRT6-Deficient Mice Drives Inflammation.Cell metabolism · 2019Article
- Article
- Prostate-specific loss of UXT promotes cancer progression.Oncotarget · 2019Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long interspersed elements, type 1(LINE-1, L1) are the most abundant and only active autonomous retrotransposons in the human genome. Native L1 elements are inefficiently expressed because of a transcription elongation defect thought to be caused by high adenosine content in L1 sequences. Previously, we constructed a highly active synthetic mouse L1 element (ORFeus-Mm), partially by reducing the nucleotide composition bias. As a result, the transcript abundance of ORFeus-Mm was greatly increased, and its retrotransposition frequency was > 200-fold higher than its native counterpart. In this paper, we report a synthetic human L1 element (ORFeus-Hs) synthesized using a similar strategy. The adenosine content of the L1 open reading frames (ORFs) was reduced from 40% to 27% by changing 25% of the bases in the ORFs, without altering the amino acid sequence. By studying a series of native/synthetic chimeric elements, we observed increased levels of full-length L1 RNA and ORF1 protein and retrotransposition frequency, mostly proportional to increased fraction of synthetic sequence. Overall, the fully synthetic ORFeus-Hs has > 40-fold more RNA but is at most only ~threefold more active than its native counterpart (L1RP); however, its absolute retrotransposition activity is similar to ORFeus-Mm. Owing to the elevated expression of the L1 RNA/protein and its high retrotransposition ability, ORFeus-Hs and its chimeric derivatives will be useful tools for mechanistic L1 studies and mammalian genome manipulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.