ArticleAnalytical chemistry2011
Supported bilayer electrophoresis under controlled buffer conditions.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Rapid Enrichment of a Native Multipass Transmembrane Protein via Cell Membrane Electrophoresis through Buffer pH and Ionic Strength Adjustment.Journal of the American Chemical Society · 2024Article
- Article
- Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers.The European physical journal. E, Soft matter · 2016Article
- Dynamic Reorganization and Correlation among Lipid Raft Components.Journal of the American Chemical Society · 2016Article
- Synthesis and Properties of Heavy Chalcogen Analogues of the Texas Reds and Related Rhodamines.Organometallics · 2014Article
- Monitoring phosphatidic acid formation in intact phosphatidylcholine bilayers upon phospholipase D catalysis.Analytical chemistry · 2014Article
- On-chip electrophoresis in supported lipid bilayer membranes achieved using low potentials.Journal of the American Chemical Society · 2014Article
- Electrophoretic measurements of lipid charges in supported bilayers.Analytical chemistry · 2013Article
- Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging.Analytical chemistry · 2013Article
- Phosphatidylserine reversibly binds Cu2+ with extremely high affinity.Journal of the American Chemical Society · 2012Article
- Protein separation by electrophoretic-electroosmotic focusing on supported lipid bilayers.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A pH controlled flow cell device was constructed to allow electrophoretic movement of charged lipids and membrane associated proteins in supported phospholipid bilayers. The device isolated electrolysis products near the electrodes from the electrophoresis process within the bilayer. This allowed the pH over the bilayer region to remain within ±0.2 pH units or better over many hours at salt concentrations up to 10 mM. Using this setup, it was found that the electrophoretic mobility of a dye conjugated lipid (Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE)) was essentially constant between pH 3.3 and 9.3. In contrast, streptavidin, which was bound to biotinylated lipids, shifted from migrating cathodically at acidic pH values to migrating anodically under basic conditions. This shift was due to the modulation of the net charge on the protein, which changed the electrophoretic forces experienced by the macromolecule. The addition of a polyethylene glycol (PEG) cushion beneath the bilayer or the increase in the ionic strength of the buffer solution resulted in a decrease of the electroosmotic force experienced by the streptavidin with little effect on the Texas Red-DHPE. As such, it was possible in part to control the electrophoretic and electroosmotic contributions to streptavidin independently of one another.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.