ArticleThe Journal of clinical investigation2011
NSR-seq transcriptional profiling enables identification of a gene signature of Plasmodium falciparum parasites infecting children.
Article in The Journal of clinical investigation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- γδ T Cells Are Required for the Induction of Sterile Immunity during Irradiated Sporozoite Vaccinations.Journal of immunology (Baltimore, Md. : 1950) · 2017Trial
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- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages.Scientific reports · 2024Article
- Plasmodium falciparum Glutamic Acid-Rich Protein-Independent Polyclonal Antibodies Inhibit Malaria Parasite Growth in Human Erythrocytes.The Journal of infectious diseases · 2024Article
- A novel computational pipeline foreLife · 2024Article
- MtrA modulates Mycobacterium tuberculosis cell division in host microenvironments to mediate intrinsic resistance and drug tolerance.Cell reports · 2023Article
- Analysis of sequence diversity in Plasmodium falciparum glutamic acid-rich protein (PfGARP), an asexual blood stage vaccine candidate.Scientific reports · 2023Article
- Transcriptome Analysis of Plasmodium falciparum Isolates From Benin Reveals Specific Gene Expression Associated With Cerebral Malaria.The Journal of infectious diseases · 2022Article
- An Invariant Protein That Colocalizes With VAR2CSA on Plasmodium falciparum-Infected Red Cells Binds to Chondroitin Sulfate A.The Journal of infectious diseases · 2022Article
- MadR mediates acyl CoA-dependent regulation of mycolic acid desaturation in mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Transcriptome signature of cell viability predicts drug response and drug interaction inCell reports methods · 2021Article
- Quantitative prediction of conditional vulnerabilities in regulatory and metabolic networks using PRIME.NPJ systems biology and applications · 2021Article
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- Common virulence gene expression in adult first-time infected malaria patients and severe cases.eLife · 2021Article
- Biofilms of the non-tuberculousCell surface (Amsterdam, Netherlands) · 2020Article
- Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment.Scientific reports · 2020Article
- Refining the transcriptome of the human malaria parasite Plasmodium falciparum using amplification-free RNA-seq.BMC genomics · 2020Article
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Authors and funding
12 authors.
Funding
Abstract
Malaria caused by Plasmodium falciparum results in approximately 1 million annual deaths worldwide, with young children and pregnant mothers at highest risk. Disease severity might be related to parasite virulence factors, but expression profiling studies of parasites to test this hypothesis have been hindered by extensive sequence variation in putative virulence genes and a preponderance of host RNA in clinical samples. We report here the application of RNA sequencing to clinical isolates of P. falciparum, using not-so-random (NSR) primers to successfully exclude human ribosomal RNA and globin transcripts and enrich for parasite transcripts. Using NSR-seq, we confirmed earlier microarray studies showing upregulation of a distinct subset of genes in parasites infecting pregnant women, including that encoding the well-established pregnancy malaria vaccine candidate var2csa. We also describe a subset of parasite transcripts that distinguished parasites infecting children from those infecting pregnant women and confirmed this observation using quantitative real-time PCR and mass spectrometry proteomic analyses. Based on their putative functional properties, we propose that these proteins could have a role in childhood malaria pathogenesis. Our study provides proof of principle that NSR-seq represents an approach that can be used to study clinical isolates of parasites causing severe malaria syndromes as well other blood-borne pathogens and blood-related diseases.
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