ArticleThe Journal of biological chemistry2011
Scm3 is a centromeric nucleosome assembly factor.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 49 citations in OpenAlex.
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- Evidence of centromeric histone 3 chaperone involved in DNA damage repair pathway in budding yeast.eLife · 2025Article
- The structure, function, and evolution of plant centromeres.Genome research · 2024Review
- The ATAD2/ANCCA homolog Yta7 cooperates with Scm3Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.eLife · 2018Article
- Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast.Nucleic acids research · 2018Article
- Histone chaperone networks shaping chromatin function.Nature reviews. Molecular cell biology · 2017Review
- Pat1 protects centromere-specific histone H3 variant Cse4 from Psh1-mediated ubiquitination.Molecular biology of the cell · 2015Article
- The centromere: epigenetic control of chromosome segregation during mitosis.Cold Spring Harbor perspectives in biology · 2014Review
- Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein.The Journal of biological chemistry · 2014Article
- Article
- Scm3 deposits a (Cse4-H4)2 tetramer onto DNA through a Cse4-H4 dimer intermediate.Nucleic acids research · 2014Article
- The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo.eLife · 2014Article
- CAL1 is the Drosophila CENP-A assembly factor.The Journal of cell biology · 2014Article
- Review
- Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition.The EMBO journal · 2013Article
- Putting CENP-A in its place.Cellular and molecular life sciences : CMLS · 2013Review
- DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast.PLoS genetics · 2013Article
- The unconventional structure of centromeric nucleosomes.Chromosoma · 2012Review
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The Cse4 nucleosome at each budding yeast centromere must be faithfully assembled each cell cycle to specify the site of kinetochore assembly and microtubule attachment for chromosome segregation. Although Scm3 is required for the localization of the centromeric H3 histone variant Cse4 to centromeres, its role in nucleosome assembly has not been tested. We demonstrate that Scm3 is able to mediate the assembly of Cse4 nucleosomes in vitro, but not H3 nucleosomes, as measured by a supercoiling assay. Localization of Cse4 to centromeres and the assembly activity depend on an evolutionarily conserved core motif in Scm3, but localization of the CBF3 subunit Ndc10 to centromeres does not depend on this motif. The centromere targeting domain of Cse4 is sufficient for Scm3 nucleosome assembly activity. Assembly does not depend on centromeric sequence. We propose that Scm3 plays an active role in centromeric nucleosome assembly.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.