ArticleJournal of bacteriology2011
Expanding the definition of class 3 inteins and their proposed phage origin.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- A Unique THN Motif Is Critical for Enabling Efficient C-Terminal Traceless Cleavage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Massive intein content inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines.Bioscience reports · 2022Article
- Conditional Alternative Protein Splicing Promoted by Inteins fromBiochemistry · 2022Article
- Inteins as Drug Targets and Therapeutic Tools.Frontiers in molecular biosciences · 2022Review
- Biochemical and Structural Characterization of an Unusual and Naturally Split Class 3 Intein.Chembiochem : a European journal of chemical biology · 2021Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms.International journal of molecular sciences · 2020Article
- Mycobacterial DnaB helicase intein as oxidative stress sensor.Nature communications · 2018Article
- Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB.Frontiers in microbiology · 2017Article
- Sequential formation of two branched intermediates during protein splicing of class three inteins.Extremophiles : life under extreme conditions · 2017Article
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- Protein splicing: how inteins escape from precursor proteins.The Journal of biological chemistry · 2014Review
- Molecular Genetics of Mycobacteriophages.Microbiology spectrum · 2014Article
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- Intermolecular domain swapping induces intein-mediated protein alternative splicing.Nature chemical biology · 2013Article
- Internal disulfide bond acts as a switch for intein activity.Biochemistry · 2013Article
- Recent progress in intein research: from mechanism to directed evolution and applications.Cellular and molecular life sciences : CMLS · 2013Review
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inteins are the protein equivalent of introns. Their protein splicing activity is essential for the host protein's maturation and function. Inteins are grouped into three classes based on sequence signature and splicing mechanism. The sequence signature of the recently characterized class 3 inteins is a noncontiguous Trp-Cys-Thr (WCT) motif and the absence of the standard class 1 Cys¹ or Ser¹ N-terminal nucleophile. The intein N-terminal Cys¹ or Ser¹ residue is essential for splicing in class 1 inteins. The mycobacteriophage Catera Gp206, Nocardioides sp. strain JS614 TOPRIM, and Thermobifida fusca YX Tfu2914 inteins have a mixture of class 1 and class 3 motifs. They carry the class 3 Trp-Cys-Thr motif and have the standard class 1 N-terminal Ser¹ or Cys¹. This study determined which class the mycobacteriophage Catera Gp206 and Nocardioides sp. JS614 TOPRIM inteins belong to based on catalytic mechanism. The mycobacteriophage Catera Gp206 intein (starting with Ser¹) is a class 3 intein, and its Ser¹ residue is not required for splicing. Based on phylogenetic analysis, we propose that class 3 inteins arose from a single mutated intein that was spread by phage into predominantly helicase genes in various phages and their hosts.
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