ArticleJournal of bacteriology2011
Lactococcus lactis ZitR is a zinc-responsive repressor active in the presence of low, nontoxic zinc concentrations in vivo.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- The MarR family regulator RmaH underlies the trade-off between acid sensitivity and antibiotic tolerance inJournal of bacteriology · 2026Article
- Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking.Chemical reviews · 2024Review
- Development of ZnSynthetic and systems biotechnology · 2024Article
- A bioinformatic analysis of zinc transporters in intestinal Lactobacillaceae.Metallomics : integrated biometal science · 2023Article
- Metallobiology of Lactobacillaceae in the gut microbiome.Journal of inorganic biochemistry · 2023Review
- Identification of a MarR Subfamily That Regulates Arsenic Resistance Genes.Applied and environmental microbiology · 2021Article
- Zinc-mediated conformational preselection mechanism in the allosteric control of DNA binding to the zinc transcriptional regulator (ZitR).Scientific reports · 2020Article
- Biophysical and structural characterization of a zinc-responsive repressor of the MarR superfamily.PloS one · 2019Article
- Tuning site-specific dynamics to drive allosteric activation in a pneumococcal zinc uptake regulator.eLife · 2018Article
- Allosteric histidine switch for regulation of intracellular zinc(II) fluctuation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Metallochaperones and metalloregulation in bacteria.Essays in biochemistry · 2017Review
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis.Microbial cell factories · 2015Article
- Characterization of Lactobacillus brevis L62 strain, highly tolerant to copper ions.World journal of microbiology & biotechnology · 2013Article
- New insights into histidine triad proteins: solution structure of a Streptococcus pneumoniae PhtD domain and zinc transfer to AdcAII.PloS one · 2013Article
- Interaction of lactic acid bacteria with metal ions: opportunities for improving food safety and quality.World journal of microbiology & biotechnology · 2012Review
- Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120.Journal of bacteriology · 2012Article
- Anaerobic p-coumarate degradation by Rhodopseudomonas palustris and identification of CouR, a MarR repressor protein that binds p-coumaroyl coenzyme A.Journal of bacteriology · 2012Article
- Metal site occupancy and allosteric switching in bacterial metal sensor proteins.Archives of biochemistry and biophysics · 2012Review
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the family Streptococcaceae, the genes encoding zinc ABC uptake systems (called zit or adc) are regulated by a coencoded MarR family member (i.e., ZitR or AdcR), whereas in the great majority of bacteria, these genes are regulated by Zur, the Fur-like zinc-responsive repressor. We studied the zit operon from Lactococcus lactis and its regulation in response to Zn(II) in vivo. zit transcription is repressed by Zn(II) in a wide concentration range starting from nontoxic micromolar levels and is derepressed at nanomolar concentrations. The level of zit promoter downregulation by environmental Zn(II) is correlated with the intracellular zinc content. The helix-turn-helix domain of ZitR is required for downregulation. In vitro, the purified protein is a dimer that complexes up to two zinc ligands per monomer and specifically binds two intact palindromic operator sites overlapping the -35 and -10 boxes of the zit promoter. DNA binding is abolished by the chelator EDTA or TPEN and fully restored by Zn(II) addition, indicating that the active repressor complexes Zn(II) with high affinity. These results suggest that derepression under starvation conditions could be an essential emergency mechanism for preserving Zn(II) homeostasis by uptake; under Zn(II)-replete conditions, the function of ZitR repression could be to help save energy rather than to avoid Zn(II) toxicity. The characterization of a MarR family zinc-responsive repressor in this report gives insight into the way Streptococcaceae efficiently adapt to Zn(II) fluctuations in their diverse ecological niches.
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