ArticleJournal of bacteriology2011
Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 85 citations in OpenAlex.
- A Spontaneous Mutation in tfoX Impairs Bioluminescence in Photobacterium kishitanii ANT-2200.Molecular microbiology · 2026Article
- Cell density and single-cell heterogeneity reveal distinct competence induction dynamics in the high-GC Gram-positive Micrococcus luteus.BMC microbiology · 2026Article
- SmallBARNA 2026: a kingdom-wide bacterial sRNA resource.Nucleic acids research · 2026Article
- DNA uptake and twitching motility are controlled by the small RNA Arp through repression of pilin translation inbioRxiv : the preprint server for biology · 2025Article
- Two transmembrane transcriptional regulators coordinate to activate chitin-induced natural transformation in Vibrio cholerae.PLoS genetics · 2025Article
- Vibrio natriegens: Application of a Fast-Growing Halophilic Bacterium.Advances in biochemical engineering/biotechnology · 2025Review
- Optimizing Conditions for the Production of Bacterial Extracellular Vesicles ofJournal of microbiology and biotechnology · 2024Article
- Diverse regulatory pathways modulate bet hedging of competence induction in epigenetically-differentiated phase variants of Streptococcus pneumoniae.Nucleic acids research · 2023Article
- Bacterial type VI secretion system (T6SS): an evolved molecular weapon with diverse functionality.Biotechnology letters · 2023Review
- Small regulatory RNAs inmicroLife · 2023Review
- Regulators of natural competence in Vibrio parahaemolyticus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- The quorum-sensing systems of Vibrio campbellii DS40M4 and BB120 are genetically and functionally distinct.Environmental microbiology · 2021Article
- Control of Competence in Vibrio fischeri.Applied and environmental microbiology · 2021Article
- Article
- Comparison of chitin-induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains.Environmental microbiology · 2020Article
- Regulation of Chitin-Dependent Growth and Natural Competence inMicroorganisms · 2020Article
- Switching fatty acid metabolism by an RNA-controlled feed forward loop.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- The quorum sensing transcription factor AphA directly regulates natural competence in Vibrio cholerae.PLoS genetics · 2019Article
- Neighbor predation linked to natural competence fosters the transfer of large genomic regions ineLife · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tfoX (also called sxy) gene product is the central regulator of DNA uptake in the naturally competent bacteria Haemophilus influenzae and Vibrio cholerae. However, the mechanisms regulating tfoX gene expression in both organisms are poorly understood. Our previous studies revealed that in V. cholerae, chitin disaccharide (GlcNAc)₂ is needed to activate the transcription and translation of V. cholerae tfoX (tfoX(VC)) to induce natural competence. In this study, we screened a multicopy library of V. cholerae DNA fragments necessary for translational regulation of tfoX(VC). A clone carrying the VC2078-VC2079 intergenic region, designated tfoR, increased the expression of a tfoX(VC)::lacZ translational fusion constructed in Escherichia coli. Using a tfoX(VC)::lacZ reporter system in V. cholerae, we confirmed that tfoR positively regulated tfoX(VC) expression at the translational level. Deletion of tfoR abolished competence for exogenous DNA even when (GlcNAc)₂ was provided. The introduction of a plasmid clone carrying the tfoR(+) gene into the tfoR deletion mutant complemented the competence deficiency. We also found that the tfoR gene encodes a 102-nucleotide small RNA (sRNA), which was transcriptionally activated in the presence of (GlcNAc)₂. Finally, we showed that this sRNA activated translation from tfoX(VC) mRNA in a highly purified in vitro translation system. Taking these results together, we propose that in the presence of (GlcNAc)₂, TfoR sRNA is expressed to activate the translation of tfoX(VC), which leads to the induction of natural competence.
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