ArticleApplied and environmental microbiology2011
Natural competence in the hyperthermophilic archaeon Pyrococcus furiosus facilitates genetic manipulation: construction of markerless deletions of genes encoding the two cytoplasmic hydrogenases.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed, 171 citations in OpenAlex.
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- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
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- Regulation of DNA Topology in Archaea: State of the Art and Perspectives.Molecular microbiology · 2025Review
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- Biotechnological Prospects ofInternational journal of molecular sciences · 2024Article
- A self-transmissible plasmid from a hyperthermophile that facilitates genetic modification of diverse Archaea.Nature microbiology · 2023Article
- Optimizing Strategies for Bio-Based Ethanol Production Using Genome-Scale Metabolic Modeling of the Hyperthermophilic Archaeon, Pyrococcus furiosus.Applied and environmental microbiology · 2023Article
- Manipulating Fermentation Pathways in the Hyperthermophilic ArchaeonApplied and environmental microbiology · 2023Article
- Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis.Molecular genetics and genomics : MGG · 2023Article
- Archaea as a Model System for Molecular Biology and Biotechnology.Biomolecules · 2023Review
- An unprecedented function for a tungsten-containing oxidoreductase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Article
- Hyper-stimulation of Pyrococcus furiosus CRISPR DNA uptake by a self-transmissible plasmid.Extremophiles : life under extreme conditions · 2022Article
- The cell biology of archaea.Nature microbiology · 2022Review
- An Abundant and Diverse New Family of Electron Bifurcating Enzymes With a Non-canonical Catalytic Mechanism.Frontiers in microbiology · 2022Article
51 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In attempts to develop a method of introducing DNA into Pyrococcus furiosus, we discovered a variant within the wild-type population that is naturally and efficiently competent for DNA uptake. A pyrF gene deletion mutant was constructed in the genome, and the combined transformation and recombination frequencies of this strain allowed marker replacement by direct selection using linear DNA. We have demonstrated the use of this strain, designated COM1, for genetic manipulation. Using genetic selections and counterselections based on uracil biosynthesis, we generated single- and double-deletion mutants of the two gene clusters that encode the two cytoplasmic hydrogenases. The COM1 strain will provide the basis for the development of more sophisticated genetic tools allowing the study and metabolic engineering of this important hyperthermophile.
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