Trial reportGut2011
A randomised trial of sheathed versus standard forceps for obtaining uncontaminated biopsy specimens of microbiota from the terminal ileum.
Trial report in Gut, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Integrative host transcriptomic and mucosal microbiome profiling reveals region-specific host-microbiome associations across the human intestine.bioRxiv : the preprint server for biology · 2026Article
- Isolation and Cultivation of Human Gut Microorganisms: A Review.Microorganisms · 2023Review
- Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine.Scientific reports · 2022Article
- Microbiota of the Oropharynx and Endoscope Compared to the Esophagus.Scientific reports · 2019Article
- Proton pump inhibitors and dysbiosis: Current knowledge and aspects to be clarified.World journal of gastroenterology · 2019Review
- Recent Advancements in Intestinal Microbiota Analyses: A Review for Non-Microbiologists.Current medical science · 2018Review
- Species Level Description of the Human Ileal Bacterial Microbiota.Scientific reports · 2018Article
- Effects of synbiotics on ileal microbiota.The Indian journal of medical research · 2018Article
- Sampling Strategies for Three-Dimensional Spatial Community Structures in IBD Microbiota Research.Frontiers in cellular and infection microbiology · 2017Review
- Association of gut microbiota with post-operative clinical course in Crohn's disease.BMC gastroenterology · 2013Article
- Total parenteral nutrition-associated lamina propria inflammation in mice is mediated by a MyD88-dependent mechanism.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Depth-dependent differences in community structure of the human colonic microbiota in health.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe study of intestinal microbiota has been revolutionised by the use of molecular methods, including terminal restriction fragment length polymorphism (T-RFLP) analysis. Microbiota studies of Crohn's disease patients have examined samples from stool or from the neoterminal ileum with a standard biopsy forceps, which could be contaminated by colonic bacteria when the forceps passes through the colonoscope channel.
objectiveTo determine whether sheathed biopsy forceps are able to obtain terminal ileal microbiota samples with less colonic bacterial contamination compared with unsheathed (standard) biopsy forceps.
designProspective randomised single-centre study. PATIENTS AND
methodsFour (paired) biopsy specimens were obtained from adjacent locations in the terminal ileum using the sheathed and standard forceps of 27 consecutive subjects undergoing colonoscopy and the microbiota were characterised using T-RFLP. The Bray-Curtis similarity index between samples (sheathed vs unsheathed forceps) was calculated within patients and significant differences were tested for across all patients.
resultsThere was not a significant difference in the microbial diversity of samples obtained using sheathed versus unsheathed forceps. The difference in microbial diversity between patients was much greater than the variability within patients by proximal versus distal site or by forceps type. LIMITATIONS: T-RFLP is based on PCR amplification, so it is not always sensitive to rare bacterial species.
conclusionStandard unsheathed forceps appear to be sufficient for microbiota sample collection from the terminal ileum.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.