ArticleJournal of lipid research2011
LC/MS/MS method for analysis of E₂ series prostaglandins and isoprostanes.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 85 citations in OpenAlex.
- Circadian Modulation of the Antioxidant Effect of Grape Consumption: A Randomized Controlled Trial.International journal of environmental research and public health · 2023Trial
- Strengthening muscle for healthy ageing: innovative treatments for sarcopenia.Nature reviews. Drug discovery · 2026Review
- Brain Adenylosuccinate Is Dramatically Increased Under Global Ischemia Without Evidence for Purine Nucleotide Cycle Activation.Journal of neurochemistry · 2025Article
- A simplified method for preventing postmortem alterations of brain prostanoids for true in situ level quantification.Journal of lipid research · 2024Article
- 12-Hydroxyeicosatetraenoic acid is the only enzymatically produced HETE increased under brain ischemia.Prostaglandins, leukotrienes, and essential fatty acids · 2024Article
- Identification and Imaging of Prostaglandin Isomers Utilizing MSJournal of the American Society for Mass Spectrometry · 2023Article
- Timapiprant, a prostaglandin D2 receptor antagonist, ameliorates pathology in a rat Alzheimer's model.Life science alliance · 2022Article
- Prostanoid Metabolites as Biomarkers in Human Disease.Metabolites · 2022Review
- Hyperhomocysteinemia dysregulates plasma levels of polyunsaturated fatty acids-derived eicosanoids.Life research · 2022Article
- Cyclooxygenase inhibition attenuates brain angiogenesis and independently decreases mouse survival under hypoxia.Journal of neurochemistry · 2021Article
- Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice-Which to Use Regarding Disease Outcomes?Antioxidants (Basel, Switzerland) · 2021Review
- Small molecules targeting cyclooxygenase/prostanoid cascade in experimental brain ischemia: Do they translate?Medicinal research reviews · 2021Review
- Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon (International journal of molecular sciences · 2021Article
- The enzymology of the human prostanoid pathway.Molecular biology reports · 2020Review
- COX-2-derived prostaglandins as mediators of the deleterious effects of nicotine in chronic kidney disease.American journal of physiology. Renal physiology · 2020Article
- Methods of the Analysis of Oxylipins in Biological Samples.Molecules (Basel, Switzerland) · 2020Review
- Novel COX-2 products ofJournal of lipid research · 2019Article
- Modern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples.Molecules (Basel, Switzerland) · 2019Review
- Directed Non-targeted Mass Spectrometry and Chemical Networking for Discovery of Eicosanoids and Related Oxylipins.Cell chemical biology · 2019Article
- Plasma Unesterified Fatty-Acid Profile Is Dramatically and Acutely Changed under Ischemic Stroke in the Mouse Model.Lipids · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
15-series prostaglandins (PGE₂s) and isoprostanes (isoPGE₂s) are robust biomarkers of oxidative stress, possess potent biological activity, and may be derived through cyclooxygenase or free radical pathways. Thus, their quantification is critical in understanding many biological processes where PG, isoPG, or oxidative stress are involved. LC/MS/MS methods allow a highly selective, sensitive, simultaneous analysis for prostanoids without derivatization. However, the LC/MS/MS methods currently used do not allow for simultaneous separation of the major brain PGE₂/D₂) and isoPGE₂ without derivatization and multiple HPLC separations. The developed LC/MS/MS method allows for the major brain PGE₂/PGD₂/isoPGE₂ such as PGE₂, entPGE₂, 8-isoPGE₂, 11β-PGE₂, PGD₂, and 15(R)-PGD₂ to be separated and quantified without derivatization. The method was validated by analyzing free and esterified isoPGE₂ in mouse brains fixed with head-focused microwave irradiation before or after global ischemia. Using the developed method, we report for the first time the esterified isoPGE₂ levels in brain tissue under basal conditions and upon global ischemia and demonstrate a nonreleasable pool of esterified isoPG upon ischemia. In addition, we demonstrated that PGE₂s found esterified in the sn-2 position in phospholipids are derived from a free radical nonenzymatic pathway under basal conditions. Our method for brain PG analysis provides a high level of selectivity to detect changes in brain PG and isoPG mass under both basal and pathological conditions.
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