ReviewTrends in biotechnology2011
Measuring enzyme activity in single cells.
Review in Trends in biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- Spatial Visualization of Secreted Lysyl Oxidase Activity from Single Living Cells via Label-Free Electrochemiluminescence Microscopy.Chemical & biomedical imaging · 2026Article
- Thin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells.Analytical chemistry · 2025Article
- Cross-Species Applications of Peptide Substrate Reporters to Quantitative Measurements of Kinase Activity.ACS measurement science au · 2024Article
- Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging.Analytical chemistry · 2024Review
- Emerging platforms for high-throughput enzymatic bioassays.Trends in biotechnology · 2023Review
- Using enzyme activities as an indicator of soil fertility in grassland - an academic dilemma.Frontiers in plant science · 2023Review
- Multienzyme activity profiling for evaluation of cell-to-cell variability of metabolic state.FASEB bioAdvances · 2022Article
- Article
- Quantifying lysosomal glycosidase activity within cells using bis-acetal substrates.Nature chemical biology · 2022Article
- Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts.ACS catalysis · 2021Article
- Photoactivatable Reporter to Perform Multiplexed and Temporally Controlled Measurements of Kinase and Protease Activity in Single Cells.Analytical chemistry · 2021Article
- A technology of a different sort: microraft arrays.Lab on a chip · 2021Review
- Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays.Journal of the American Chemical Society · 2020Article
- Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.Journal of Cancer · 2020Article
- Label-Free Assay of Protein Tyrosine Phosphatase Activity in Single Cells.Analytical chemistry · 2019Article
- Population-based analysis of cell-penetrating peptide uptake using a microfluidic droplet trapping array.Analytical and bioanalytical chemistry · 2019Article
- CPProtectides: Rapid uptake of well-folded β-hairpin peptides with enhanced resistance to intracellular degradation.Peptide science (Hoboken, N.J.) · 2019Article
- Electron microscopy snapshots of single particles from single cells.The Journal of biological chemistry · 2019Article
- Selection and optimization of enzyme reporters for chemical cytometry.Methods in enzymology · 2019Article
- Chemical fixation to arrest phospholipid signaling for chemical cytometry.Journal of chromatography. A · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Seemingly identical cells can differ in their biochemical state, function and fate, and this variability plays an increasingly recognized role in organism-level outcomes. Cellular heterogeneity arises in part from variation in enzyme activity, which results from interplay between biological noise and multiple cellular processes. As a result, single-cell assays of enzyme activity, particularly those that measure product formation directly, are crucial. Recent innovations have yielded a range of techniques to obtain these data, including image-, flow- and separation-based assays. Research to date has focused on easy-to-measure glycosylases and clinically-relevant kinases. Expansion of these techniques to a wider range and larger number of enzymes will answer contemporary questions in proteomics and glycomics, specifically with respect to biological noise and cellular heterogeneity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.