ArticleBiomaterials2011
Engineering spatial control of multiple differentiation fates within a stem cell population.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 107 citations in OpenAlex.
- Electrical Signals at the Subcellular Scale: How Electroactive Materials Regulate Stem Cell Fate.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Immortalized Rat Tendon-Derived Stem Cells for Tendon Tissue Engineering.Bioengineering (Basel, Switzerland) · 2026Article
- A Heparin-Functionalized Scaffold with HB-EGF Immobilization for Tissue Engineering.Advanced healthcare materials · 2026Article
- The Characterization of Serum-Free Media on Human Mesenchymal Stem Cell Fibrochondrogenesis.Bioengineering (Basel, Switzerland) · 2025Article
- Hierarchy Reproduction: Multiphasic Strategies for Tendon/Ligament-Bone Junction Repair.Biomaterials research · 2025Review
- Facile and rapid fabrication of a novel 3D-printable, visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair.Bioactive materials · 2024Article
- Progress in bioprinting technology for tissue regeneration.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2023Review
- Effects of Myostatin on Nuclear Morphology at the Myotendinous Junction.International journal of molecular sciences · 2023Article
- Clinical perspectives for repairing rotator cuff injuries with multi-tissue regenerative approaches.Journal of orthopaedic translation · 2022Review
- Hybprinting for musculoskeletal tissue engineering.iScience · 2022Review
- Laminin 411 mediates endothelial specification via multiple signaling axes that converge on β-catenin.Stem cell reports · 2022Article
- Spatial Stem Cell Fate Engineering via Facile Morphogen Localization.Advanced healthcare materials · 2021Article
- Cell trafficking and regulation of osteoblastogenesis by extracellular vesicle associated bone morphogenetic protein 2.Journal of extracellular vesicles · 2021Article
- 3D Bioprinting of Human Tissues: Biofabrication, Bioinks, and Bioreactors.International journal of molecular sciences · 2021Review
- Engineering Musculoskeletal Grafts for Multi-Tissue Unit Repair: Lessons From Developmental Biology and Wound Healing.Frontiers in physiology · 2021Review
- Growth Factor Roles in Soft Tissue Physiology and Pathophysiology.Advances in experimental medicine and biology · 2021Article
- Concurrent multi-lineage differentiation of mesenchymal stem cells through spatial presentation of growth factors.Biomedical materials (Bristol, England) · 2020Article
- Tough and tunable scaffold-hydrogel composite biomaterial for soft-to-hard musculoskeletal tissue interfaces.Science advances · 2020Article
- Reversible control of biomaterial properties for dynamically tuning cell behavior.Journal of applied polymer science · 2020Article
- Acoustic Patterning of Growth Factor for Three-Dimensional Tissue Engineering.Tissue engineering. Part A · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The capability to engineer microenvironmental cues to direct a stem cell population toward multiple fates, simultaneously, in spatially defined regions is important for understanding the maintenance and repair of multi-tissue units. We have previously developed an inkjet-based bioprinter to create patterns of solid-phase growth factors (GFs) immobilized to an extracellular matrix (ECM) substrate, and applied this approach to drive muscle-derived stem cells toward osteoblasts 'on-pattern' and myocytes 'off-pattern' simultaneously. Here this technology is extended to spatially control osteoblast, tenocyte and myocyte differentiation simultaneously. Utilizing immunofluorescence staining to identify tendon-promoting GFs, fibroblast growth factor-2 (FGF-2) was shown to upregulate the tendon marker Scleraxis (Scx) in C3H10T1/2 mesenchymal fibroblasts, C2C12 myoblasts and primary muscle-derived stem cells, while downregulating the myofibroblast marker α-smooth muscle actin (α-SMA). Quantitative PCR studies indicated that FGF-2 may direct stem cells toward a tendon fate via the Ets family members of transcription factors such as pea3 and erm. Neighboring patterns of FGF-2 and bone morphogenetic protein-2 (BMP-2) printed onto a single fibrin-coated coverslip upregulated Scx and the osteoblast marker ALP, respectively, while non-printed regions showed spontaneous myotube differentiation. This work illustrates spatial control of multi-phenotype differentiation and may have potential in the regeneration of multi-tissue units.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.