ArticleJournal of molecular biology2011
Consequences of the overexpression of a eukaryotic membrane protein, the human KDEL receptor, in Escherichia coli.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
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- Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs.ACS synthetic biology · 2022Article
- Developing a dynamic equilibrium system in Escherichia coli to improve the production of recombinant proteins.Applied microbiology and biotechnology · 2022Article
- Strategies for Successful Over-Expression of Human Membrane Transport Systems Using Bacterial Hosts: Future Perspectives.International journal of molecular sciences · 2022Review
- Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Expression Optimizing of Recombinant Oxalyl-CoA Decarboxylase inAdvanced biomedical research · 2022Article
- Increased Synthesis of a Magnesium Transporter MgtA During Recombinant Autotransporter Expression in Escherichia coli.Applied biochemistry and biotechnology · 2021Article
- Engineering of Challenging G Protein-Coupled Receptors for Structure Determination and Biophysical Studies.Molecules (Basel, Switzerland) · 2021Review
- Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii.Microbial cell factories · 2021Article
- How Quality Control Systems AID Sec-Dependent Protein Translocation.Frontiers in molecular biosciences · 2021Review
- Functionality of membrane proteins overexpressed and purified from E. coli is highly dependent upon the strain.Scientific reports · 2019Article
- Mutations in sigma 70 transcription factor improves expression of functional eukaryotic membrane proteins in Escherichia coli.Scientific reports · 2019Article
- Expression of Leptospira membrane proteins Signal Peptidase (SP) and Leptospira Endostatin like A (Len A) in BL-21(DE3) is toxic to the host cells.Journal, genetic engineering & biotechnology · 2018Article
- Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress.Applied and environmental microbiology · 2018Article
- The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli.Microbial cell factories · 2017Article
- On the Spatial Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host Overexpressing Membrane Proteins.PLoS genetics · 2016Article
- A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution.Scientific reports · 2016Article
- Method to increase the yield of eukaryotic membrane protein expression in Saccharomyces cerevisiae for structural and functional studies.Protein science : a publication of the Protein Society · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Escherichia coli is the most widely used host for producing membrane proteins. Thus far, to study the consequences of membrane protein overexpression in E. coli, we have focussed on prokaryotic membrane proteins as overexpression targets. Their overexpression results in the saturation of the Sec translocon, which is a protein-conducting channel in the cytoplasmic membrane that mediates both protein translocation and insertion. Saturation of the Sec translocon leads to (i) protein misfolding/aggregation in the cytoplasm, (ii) impaired respiration, and (iii) activation of the Arc response, which leads to inefficient ATP production and the formation of acetate. The overexpression yields of eukaryotic membrane proteins in E. coli are usually much lower than those of prokaryotic ones. This may be due to differences between the consequences of the overexpression of prokaryotic and eukaryotic membrane proteins in E. coli. Therefore, we have now also studied in detail how the overexpression of a eukaryotic membrane protein, the human KDEL receptor, affects E. coli. Surprisingly, the consequences of the overexpression of a prokaryotic and a eukaryotic membrane protein are very similar. Strain engineering and likely also protein engineering can be used to remedy the saturation of the Sec translocon upon overexpression of both prokaryotic and eukaryotic membrane proteins in E. coli.
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