ArticleJournal of controlled release : official journal of the Controlled Release Society2011
Selective gene transfection of individual cells in vitro with plasmonic nanobubbles.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 79 citations in OpenAlex.
- A Permeabilization Workflow To Enable Specific Multiplexed Profiling Using SERS Nanoparticles.ACS applied materials & interfaces · 2025Article
- Emerging nanocarriers as advanced delivery tools for the treatment of leukemia.Nanomedicine (London, England) · 2025Review
- Spatially-Resolved Organoid Transfection by Porous Silicon-Mediated Optoporation.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- A Systematic Study on Long-acting Nanobubbles: Current Advancement and Prospects on Theranostic Properties.Advanced pharmaceutical bulletin · 2024Review
- Cas9 RNP transfection by vapor nanobubble photoporation forMolecular therapy. Nucleic acids · 2021Article
- Enhancements of Cancer Cell Damage Efficiencies in Photothermal and Photodynamic Processes through Cell Perforation and Preheating with Surface Plasmon Resonance of Gold Nanoring.Molecules (Basel, Switzerland) · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation.International journal of nanomedicine · 2017Article
- Reversible control of current across lipid membranes by local heating.Scientific reports · 2016Article
- All-in-one processing of heterogeneous human cell grafts for gene and cell therapy.Molecular therapy. Methods & clinical development · 2016Article
- Spatial temperature mapping within polymer nanocomposites undergoing ultrafast photothermal heating via gold nanorods.Nanoscale · 2014Article
- Sonoporation-enhanced chemotherapy significantly reduces primary tumour burden in an orthotopic pancreatic cancer xenograft.Molecular imaging and biology · 2014Article
- Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells.Biomedical optics express · 2013Article
- Gold nanoparticle mediated laser transfection for efficient siRNA mediated gene knock down.PloS one · 2013Article
- Towards theranostic multicompartment microcapsules: in-situ diagnostics and laser-induced treatment.Theranostics · 2013Review
- Cell-specific multifunctional processing of heterogeneous cell systems in a single laser pulse treatment.ACS nano · 2012Article
- Plasmonic nanobubbles enhance efficacy and selectivity of chemotherapy against drug-resistant cancer cells.Advanced materials (Deerfield Beach, Fla.) · 2012Article
- Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles.Biomaterials · 2012Article
- Plasmonic nanobubble-enhanced endosomal escape processes for selective and guided intracellular delivery of chemotherapy to drug-resistant cancer cells.Biomaterials · 2012Article
- New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization.International journal of nanomedicine · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Gene delivery and transfection of eukaryotic cells are widely used for research and for developing gene cell therapy. However, the existing methods lack selectivity, efficacy and safety when heterogeneous cell systems must be treated. We report a new method that employs plasmonic nanobubbles (PNBs) for delivery and transfection. A PNB is a novel, tunable cellular agent with a dual mechanical and optical action due to the formation of the vapor nanobubble around a transiently heated gold nanoparticle upon its exposure to a laser pulse. PNBs enabled the mechanical injection of the extracellular cDNA plasmid into the cytoplasm of individual target living cells, cultured leukemia cells and human CD34+ CD117+ stem cells and expression of a green fluorescent protein (GFP) in those cells. PNB generation and lifetime correlated with the expression of green fluorescent protein in PNB-treated cells. Optical scattering by PNBs additionally provided the detection of the target cells and the guidance of cDNA injection at single cell level. In both cell models PNBs demonstrated a gene transfection effect in a single pulse treatment with high selectivity, efficacy and safety. Thus, PNBs provided targeted gene delivery at the single cell level in a single pulse procedure that can be used for safe and effective gene therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.