ArticleBioconjugate chemistry2011
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots.
Article in Bioconjugate chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- An engineered redox-switchable streptavidin mutein enables the high-affinity capture and efficient elution of biotinylated ligands.Protein science : a publication of the Protein Society · 2025Article
- Quantum Dot-based Bio-conjugates as an Emerging Bioimaging Tool for Cancer Theranostic- A Review.Current drug targets · 2024Review
- Article
- Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta.ACS omega · 2022Article
- Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation.Scientific reports · 2022Article
- Nanobiosensor Based on Sugar Code-AuNPs Aggregation: A Key to Opening New Gates in Rapid Diagnosis of Streptococcal Pharyngitis.Frontiers in bioengineering and biotechnology · 2022Article
- A Novel Biotinylated Homotryptamine Derivative for Quantum Dot Imaging of Serotonin Transporter in Live Cells.Frontiers in cellular neuroscience · 2021Article
- Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Engineering magnetic nanoparticles and their integration with microfluidics for cell isolation.Journal of colloid and interface science · 2020Article
- Sugar Codes Conjugated Alginate: An Innovative Platform to Make a Strategic Breakthrough in Simultaneous Prophylaxis of GERD andDrug design, development and therapy · 2020Article
- Single-Molecule Flow Platform for the Quantification of Biomolecules Attached to Single Nanoparticles.Analytical chemistry · 2018Article
- Structural Contributions to Hydrodynamic Diameter for Quantum Dots Optimized for Live-Cell Single-Molecule Tracking.The journal of physical chemistry. C, Nanomaterials and interfaces · 2018Article
- Supramolecular Host-Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles.ACS omega · 2017Article
- Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS.Journal of physics D: Applied physics · 2017Review
- NeutrAvidin Functionalization of CdSe/CdS Quantum Nanorods and Quantification of Biotin Binding Sites using Biotin-4-Fluorescein Fluorescence Quenching.Bioconjugate chemistry · 2016Article
- Multiplexed modular genetic targeting of quantum dots.ACS nano · 2014Article
- Plug-and-play pairing via defined divalent streptavidins.Journal of molecular biology · 2014Article
- Impacts of quantum dots in molecular detection and bioimaging of cancer.BioImpacts : BI · 2014Review
- Article
- Multi-color quantum dot tracking using a high-speed hyperspectral line-scanning microscope.PloS one · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to their performance in cell tagging, tracking, and imaging experiments. Commercially available streptavidin conjugates (SAv QDs) are the most commonly used tool for preparing QD-biomolecule conjugates. The fluorescence quenching of biotin-4-fluorscein (B4F) provides a straightforward assay to quantify the number of biotin binding sites per SAv QD. The utility of this method was demonstrated by quantitatively characterizing the biotin binding capacity of commercially available amphiphilic poly(acrylic acid) Qdot ITK SAv conjugates and poly(ethylene glycol) modified Qdot PEG SAv conjugates with emission wavelengths of 525, 545, 565, 585, 605, 625, 655, 705, and 800 nm. Results showed that 5- to 30-fold more biotin binding sites are available on ITK SAv QDs compared to PEG SAv QDs of the same color with no systematic variation of biotin binding capacity with size.
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Registered trials
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