ArticleJournal of tissue engineering and regenerative medicine2012
The small molecule PKA-specific cyclic AMP analogue as an inducer of osteoblast-like cells differentiation and mineralization.
Article in Journal of tissue engineering and regenerative medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Modulation of Stress and Anabolic Signalling Pathways by Whey Protein Isolate in C2C12 Cells Under Exercise-Mimetic Conditions.Biomedicines · 2026Article
- Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia.Nature communications · 2025Article
- Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells.Scientific reports · 2025Article
- The Use of Small-Molecule Compounds for Cell Adhesion and Migration in Regenerative Medicine.Biomedicines · 2023Review
- Regenerative engineering of long bones using the small molecule forskolin.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Involvement of the TNF-α/SATB2 axis in the induced apoptosis and inhibited autophagy of osteoblasts by the antipsychotic Risperidone.Molecular medicine (Cambridge, Mass.) · 2022Article
- The Marine-Derived Natural Product Epiloliolide Isolated fromMarine drugs · 2021Article
- Transforming growth factor-β1-induced N-cadherin drives cell-cell communication through connexin43 in osteoblast lineage.International journal of oral science · 2021Article
- Reprogrammed mRNA translation drives resistance to therapeutic targeting of ribosome biogenesis.The EMBO journal · 2020Article
- Is NO the Answer? The Nitric Oxide Pathway Can Support Bone Morphogenetic Protein 2 Mediated Signaling.Cells · 2019Article
- Pinoresinol promotes MC3T3‑E1 cell proliferation and differentiation via the cyclic AMP/protein kinase A signaling pathway.Molecular medicine reports · 2019Article
- Current advances for bone regeneration based on tissue engineering strategies.Frontiers of medicine · 2019Review
- Pulsed electromagnetic fields: promising treatment for osteoporosis.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2019Review
- Harnessing cAMP signaling in musculoskeletal regenerative engineering.Drug discovery today · 2017Review
- Poly (lactic acid)-based biomaterials for orthopaedic regenerative engineering.Advanced drug delivery reviews · 2016Review
- One-day treatment of small molecule 8-bromo-cyclic AMP analogue induces cell-based VEGF production for in vitro angiogenesis and osteoblastic differentiation.Journal of tissue engineering and regenerative medicine · 2016Article
- Small molecule-driven direct conversion of human pluripotent stem cells into functional osteoblasts.Science advances · 2016Article
- Linarin promotes osteogenic differentiation by activating the BMP-2/RUNX2 pathway via protein kinase A signaling.International journal of molecular medicine · 2016Article
- Small molecule delivery through nanofibrous scaffolds for musculoskeletal regenerative engineering.Nanomedicine : nanotechnology, biology, and medicine · 2014Review
- Pituitary adenylate cyclase-activating polypeptide (PACAP) signalling enhances osteogenesis in UMR-106 cell line.Journal of molecular neuroscience : MN · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Osteoblastic differentiation is an important landmark for bone formation, bone repair and regeneration; however, it is a very complex process controlled by different signalling mechanisms. Several groups have reported that the cyclic adenosine monophosphate (cAMP) signalling system is responsible for regulating osteoblast cell differentiation. Nonetheless, to date, the principle role of the cAMP molecules related to this process remains controversial. Moreover, the underlying cAMP-dependent signalling cascade governing the osteoblastic differentiation has not been clarified. In this study we investigated the roles of the cAMP-dependent protein kinase A (PKA) signalling in proliferation, differentiation and mineralization of osteoblast-like MC3T3-E1 cells, using the PKA-specific small molecule cAMP analogue, 6-Bnz-cAMP, at 100 µM. Alkaline phosphatase (ALP) activity, runt transcription factor 2 (Runx2), osteopontin (OPN) and osteocalcin (OCN) protein expressions were used as osteoblast-specific markers to demonstrate osteoblastic differentiation. Further, calcium measurement of the extracellular matrix was employed as the hallmark of matrix mineralization or calcification. We report here that activation of PKA by the small molecule 6-Bnz-cAMP induces osteoblastic differentiation and matrix mineralization of osteoblast-like MC3T3-E1 cells. Moreover, 6-Bnz-cAMP does not induce cytotoxicity to the cells, as revealed by our cell proliferation studies. Therefore, based on these findings, we propose that the PKA-specific small molecule 6-Bnz-cAMP may serve as a novel bone-inducing growth factor for repairing and regenerating bone tissues during bone-regenerative engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.