ArticleSoft matter2009
An ion-channel-containing model membrane: structural determination by magnetic contrast neutron reflectometry.
Article in Soft matter, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Improving polarized neutron reflectometry experiments on soft-matter samples: optimization of the solid substrate structure.Journal of applied crystallography · 2026Article
- Twin-compartment solid-liquid cells for neutron reflectometry.Journal of applied crystallography · 2026Article
- Microscopic insight into HIV fusion peptide-mediated dehydration and packing regulation in membranes.Biophysical journal · 2025Article
- Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Probing the Separation Distance between Biological Nanoparticles and Cell Membrane Mimics Using Neutron Reflectometry with Sub-Nanometer Accuracy.Journal of the American Chemical Society · 2022Article
- Synchrotron tomography applications in agriculture and food sciences research: a review.Plant methods · 2022Review
- Exploiting neutron scattering contrast variation in biological membrane studies.Biophysics reviews · 2022Review
- Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding.Biophysical journal · 2021Article
- Biomembrane Structure and Material Properties Studied With Neutron Scattering.Frontiers in chemistry · 2021Review
- Studying the surfaces of bacteria using neutron scattering: finding new openings for antibiotics.Biochemical Society transactions · 2020Review
- The Location of the Antimicrobial Peptide Maculatin 1.1 in Model Bacterial Membranes.Frontiers in chemistry · 2020Article
- Nuclear Spin Incoherent Neutron Scattering from Quantum Well Resonators.Physical review letters · 2019Article
- Physical Properties of Bacterial Outer Membrane Models: Neutron Reflectometry & Molecular Simulation.Biophysical journal · 2019Article
- Optimization of reflectometry experiments using information theory.Journal of applied crystallography · 2019Article
- Non-lamellar lipid assembly at interfaces: controlling layer structure by responsive nanogel particles.Interface focus · 2017Article
- Effect of the CRAC Peptide, VLNYYVW, on mPTP Opening in Rat Brain and Liver Mitochondria.International journal of molecular sciences · 2016Article
- An Accurate In Vitro Model of theAngewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2015Article
- An accurate in vitro model of the E. coli envelope.Angewandte Chemie (International ed. in English) · 2015Article
- Interrogating protonated/deuterated fibronectin fragment layers adsorbed to titania by neutron reflectivity and their concomitant control over cell adhesion.Journal of the Royal Society, Interface · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
To many biophysical characterisation techniques, biological membranes appear as two-dimensional structures with details of their third dimension hidden within a 5 nm profile. Probing this structure requires methods able to discriminate multiple layers a few Ångströms thick. Given sufficient resolution, neutron methods can provide the required discrimination between different biochemical components, especially when selective deuteration is employed. We have used state-of-the-art neutron reflection methods, with resolution enhancement via magnetic contrast variation to study an oriented model membrane system. The model is based on the Escherichia coli outer membrane protein OmpF fixed to a gold surface via an engineered cysteine residue. Below the gold is buried a magnetic metal layer which, in a magnetic field, displays different scattering strengths to spin-up and spin-down neutrons. This provides two independent datasets from a single biological sample. Simultaneous fitting of the two datasets significantly refines the resulting model. A β-mercaptoethanol (βME) passivating surface, applied to the gold to prevent protein denaturation, is resolved for the first time as an 8.2 ± 0.6 Å thick layer, demonstrating the improved resolution and confirming that this layer remains after OmpF assembly. The thiolipid monolayer (35.3 ± 0.5 Å), assembled around the OmpF is determined and finally a fluid DMPC layer is added (total lipid thickness 58.7 ± 0.9 Å). The dimensions of trimeric OmpF in isolation (53.6 ± 2.5 Å), after assembly of lipid monolayer (57.5 ± 0.9 Å) and lipid bilayer (58.7 ± 0.9 Å), are precisely determined and show little variation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.