ArticleBiology of reproduction2011
Testicular lumicrine factors regulate ERK, STAT, and NFKB pathways in the initial segment of the rat epididymis to prevent apoptosis.
Article in Biology of reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Extracellular Vesicles, Nutrient Balance, and Redox Metabolism During Epididymal Sperm Maturation: A Perspective on the Complexity of Intercellular Interplay.Advances in anatomy, embryology, and cell biology · 2026Review
- Distinct actions of testicular endocrine and lumicrine signaling on the proximal epididymal transcriptome.Reproductive biology and endocrinology : RB&E · 2024Article
- Busulfan administration replicated the characteristics of the epididymal initial segment observed in mice lacking testis-epididymis lumicrine signaling.The Journal of reproduction and development · 2024Article
- Epithelial and mesenchymal fate decisions in Wolffian duct development.Trends in endocrinology and metabolism: TEM · 2023Review
- Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls' testes and epididymides.Veterinarni medicina · 2023Article
- Effect of C-Type Natriuretic Peptide (CNP) on Spermatozoa Maturation in Adult Rat Epididymis.Current issues in molecular biology · 2023Article
- The MAPK/ERK signaling pathway regulates the expression and localization of Cx43 in mouse proximal epididymis†.Biology of reproduction · 2022Article
- Seasonal expression of extracellular signal regulated kinases in the colon of wild ground squirrels (Spermophilus dauricus).Molecular biology reports · 2022Article
- Development and Differentiation of Epididymal Epithelial Cells in Korean Native Black Goat.Animals : an open access journal from MDPI · 2020Article
- Androgens are essential for epithelial cell recovery after efferent duct ligation in the initial segment of the mouse epididymis†.Biology of reproduction · 2020Article
- Epithelial dynamics in the epididymis: role in the maturation, protection, and storage of spermatozoa.Andrology · 2019Review
- Testis Transcriptome Modulation in Klinefelter Patients with Hypospermatogenesis.Scientific reports · 2017Article
- Initial Segment Differentiation Begins During a Critical Window and Is Dependent upon Lumicrine Factors and SRC Proto-Oncogene (SRC) in the Mouse.Biology of reproduction · 2016Article
- Is the Epididymis a Series of Organs Placed Side By Side?Biology of reproduction · 2016Review
- Tyrosine kinase-mediated axial motility of basal cells revealed by intravital imaging.Nature communications · 2016Article
- The MAPK/ERK-Signaling Pathway Regulates the Expression and Distribution of Tight Junction Proteins in the Mouse Proximal Epididymis.Biology of reproduction · 2016Article
- Role of testicular luminal factors on Basal cell elongation and proliferation in the mouse epididymis.Biology of reproduction · 2015Article
- PTEN signaling through RAF1 proto-oncogene serine/threonine kinase (RAF1)/ERK in the epididymis is essential for male fertility.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- ROS1 signaling regulates epithelial differentiation in the epididymis.Endocrinology · 2014Article
- PE‑induced apoptosis in SMMC‑7721 cells: involvement of Erk and Stat signalling pathways.International journal of molecular medicine · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The initial segment of the epididymis is vital for male fertility; therefore, it is important to understand the mechanisms that regulate this important region. Deprival of testicular luminal fluid factors/lumicrine factors from the epididymis results in a wave of apoptosis in the initial segment. In this study, a combination of protein array and microarray analyses was used to examine the early changes in downstream signal transduction pathways following loss of lumicrine factors. We discovered the following cascade of events leading to the loss of protection and eventual apoptosis: in the first 6 h after loss of lumicrine factors, down-regulation of the ERK pathway components was observed at the mRNA expression and protein activity levels. Microarray analysis revealed that mRNA levels of several key components of the ERK pathway, Dusp6, Dusp5, and Etv5, decreased sharply, while the analysis from the protein array revealed a decline in the activities of MAP2K1/2 and MAPK1. Immunostaining of phospho-MAPK3/1 indicated that down-regulation of the ERK pathway was specific to the epithelial cells of the initial segment. Subsequently, after 12 h of loss of lumicrine factors, levels of mRNA expression of STAT and NFKB pathway components increased, mRNA levels of several genes encoding cell cycle inhibitors increased, and levels of protein expression of several proapoptotic phosphatases increased. Finally, after 18 h of loss of protection from lumicrine factors, apoptosis was observed. In conclusion, testicular lumicrine factors protect the cells of the initial segment by activating the ERK pathway, repressing STAT and NFKB pathways, and thereby preventing apoptosis.
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