ArticleThe Journal of biological chemistry2011
Genome-wide analysis of transcription factor E2F1 mutant proteins reveals that N- and C-terminal protein interaction domains do not participate in targeting E2F1 to the human genome.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 52 citations in OpenAlex.
- PKMYT1 Is a Marker of Treatment Response and a Therapeutic Target for CDK4/6 Inhibitor-Resistance in ER+ Breast Cancer.Molecular cancer therapeutics · 2024Article
- The pRb/RBL2-E2F1/4-GCN5 axis regulates cancer stem cell formation and G0 phase entry/exit by paracrine mechanisms.Nature communications · 2024Article
- DNA G-Quadruplex in NRP1 Promoter Facilitates SARS-CoV-2 Infection.International journal of molecular sciences · 2024Article
- RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling.Cell reports · 2023Article
- Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer.Cancers · 2022Article
- Bioinformatic-based mechanism identification ofJournal of gastrointestinal oncology · 2022Article
- Variational autoencoding of gene landscapes during mouse CNS development uncovers layered roles of Polycomb Repressor Complex 2.Nucleic acids research · 2022Article
- E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.Nature communications · 2021Article
- Therapeutic Targeting ofAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes.Scientific reports · 2018Article
- Identification of Drivers of Aneuploidy in Breast Tumors.Cell reports · 2018Article
- Differential impact of RB status on E2F1 reprogramming in human cancer.The Journal of clinical investigation · 2018Article
- Rb family proteins enforce the homeostasis of quiescent hematopoietic stem cells by repressing Socs3 expression.The Journal of experimental medicine · 2017Article
- Coordination of Myeloid Differentiation with Reduced Cell Cycle Progression by PU.1 Induction of MicroRNAs Targeting Cell Cycle Regulators and Lipid Anabolism.Molecular and cellular biology · 2017Article
- E2F1 mediates sustained lipogenesis and contributes to hepatic steatosis.The Journal of clinical investigation · 2016Article
- Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression.Nature communications · 2015Article
- Structural insights into the DNA-binding specificity of E2F family transcription factors.Nature communications · 2015Article
- Regulation of E2 promoter binding factor 1 (E2F1) transcriptional activity through a deubiquitinating enzyme, UCH37.The Journal of biological chemistry · 2015Article
- The TCF C-clamp DNA binding domain expands the Wnt transcriptome via alternative target recognition.Nucleic acids research · 2014Article
- Leveraging biological replicates to improve analysis in ChIP-seq experiments.Computational and structural biotechnology journal · 2014Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Previous studies of E2F family members have suggested that protein-protein interactions may be the mechanism by which E2F proteins are recruited to specific genomic regions. We have addressed this hypothesis on a genome-wide scale using ChIP-seq analysis of MCF7 cell lines that express tagged wild type and mutant E2F1 proteins. First, we performed ChIP-seq for tagged WT E2F1. Then, we analyzed E2F1 proteins that lacked the N-terminal SP1 and cyclin A binding domains, the C-terminal transactivation and pocket protein binding domains, and the internal marked box domain. Surprisingly, we found that the ChIP-seq patterns of the mutant proteins were identical to that of WT E2F1. However, mutation of the DNA binding domain abrogated all E2F1 binding to the genome. These results suggested that the interaction between the E2F1 DNA binding domain and a consensus motif may be the primary determinant of E2F1 recruitment. To address this possibility, we analyzed the in vivo binding sites for the in vitro-derived consensus E2F1 motif (TTTSSCGC) and also performed de novo motif analysis. We found that only 12% of the ChIP-seq peaks contained the TTTSSCGC motif. De novo motif analysis indicated that most of the in vivo sites lacked the 5' half of the in vitro-derived consensus, having instead the in vivo consensus of CGCGC. In summary, our findings do not provide support for the model that protein-protein interactions are involved in recruiting E2F1 to the genome, but rather suggest that recognition of a motif found at most human promoters is the critical determinant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.