ArticleThe Plant journal : for cell and molecular biology2011
A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii.
Article in The Plant journal : for cell and molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
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Who cites it
181 citing papers in PubMed, 363 citations in OpenAlex.
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- SAGA1 and SAGA2 localize the starch sheath to the pyrenoid inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.Nature cell biology · 2026Article
- Investigating overflow metabolism in heterotrophic cultures of the green alga Chromochloris zofingiensis.Metabolic engineering · 2026Article
- Artificial Symbiosis for Bulk Production of Bacterial Cellulose Composites.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- SAGA1 and SAGA2 localize the starch sheath to the pyrenoid inbioRxiv : the preprint server for biology · 2026Article
- Article
- Single-cell ionomes of terrestrial cryosphere algae.Communications earth & environment · 2026Article
- Mono-mix strategy enables comparative proteomics of a cross-kingdom microbial symbiosis.PloS one · 2026Article
- Hidden diversity: Transcriptomic and photosynthetic variation among common 'wild type' Chlamydomonas strains.The Plant journal : for cell and molecular biology · 2025Article
- Targeted genetic manipulation and yeast-like evolutionary genomics in the green alga Auxenochlorella.The Plant cell · 2025Article
- Targeted genetic manipulation and yeast-like evolutionary genomics in the green algabioRxiv : the preprint server for biology · 2025Article
- Investigating Overflow Metabolism in Heterotrophic Cultures of the Green AlgabioRxiv : the preprint server for biology · 2025Article
- Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.bioRxiv : the preprint server for biology · 2025Article
- An Algal Nutrient-Replete, Optimized Medium for Fast Growth and High Triacylglycerol Accumulation.Plant direct · 2025Article
- Preparation, maintenance and propagation of synchronous cultures of photoactive Chlamydomonas cells.Nature protocols · 2025Review
121 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Interest in exploiting algae as a biofuel source and the role of inorganic nutrient deficiency in inducing triacylglyceride (TAG) accumulation in cells necessitates a strategy to efficiently formulate species-specific culture media that can easily be manipulated. Using the reference organism Chlamydomonas reinhardtii, we tested the hypothesis that modeling trace element supplements after the cellular ionome would result in optimized cell growth. We determined the trace metal content of several commonly used Chlamydomonas strains in various culture conditions and developed a revised trace element solution to parallel these measurements. Comparison of cells growing in the revised supplement versus a traditional trace element solution revealed faster growth rates and higher maximum cell densities with the revised recipe. RNA-seq analysis of cultures growing in the traditional versus revised medium suggest that the variation in transcriptomes was smaller than that found between different wild-type strains grown in traditional Hutner's supplement. Visual observation did not reveal defects in cell motility or mating efficiency in the new supplement. Ni²⁺-inducible expression from the CYC6 promoter remained a useful tool, albeit with an increased requirement for Ni²⁺ because of the introduction of an EDTA buffer system in the revised medium. Other advantages include more facile preparation of trace element stock solutions, a reduction in total chemical use, a more consistent batch-to-batch formulation and long-term stability (tested up to 5 years). Under the new growth regime, we analyzed cells growing under different macro- and micronutrient deficiencies. TAG accumulation in N deficiency is comparable in the new medium. Fe and Zn deficiency also induced TAG accumulation, as suggested by Nile Red staining. This approach can be used to efficiently optimize culture conditions for other algal species to improve growth and to assay cell physiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.