ArticleAnalytical chemistry2011
Coupling sodium dodecyl sulfate-capillary polyacrylamide gel electrophoresis with matrix-assisted laser desorption ionization time-of-flight mass spectrometry via a poly(tetrafluoroethylene) membrane.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Rapid identification of antibody impurities in size-based electrophoresis via CZE-MS generated spectral library.Scientific reports · 2024Article
- Combination of strong anion exchange liquid chromatography with microchip capillary electrophoresis sodium dodecyl sulfate for rapid two-dimensional separations of complex protein mixtures.Analytical and bioanalytical chemistry · 2022Article
- Recent Advances in Porphyrin-Based Materials for Metal Ions Detection.International journal of molecular sciences · 2020Review
- Titanium Dioxide Photocatalytic Polymerization of Acrylamide for Gel Electrophoresis (TIPPAGE) of Proteins and Structural Identification by Mass Spectrometry.Scientific reports · 2016Article
- Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis.Analytical methods : advancing methods and applications · 2014Article
- Recent advances in coupling capillary electrophoresis-based separation techniques to ESI and MALDI-MS.Electrophoresis · 2014Review
- Performing isoelectric focusing and simultaneous fractionation of proteins on a rotary valve followed by sodium dodecyl-polyacrylamide gel electrophoresis.Analytical chemistry · 2013Article
- Western blotting using microchip electrophoresis interfaced to a protein capture membrane.Analytical chemistry · 2013Article
- Chip-capillary hybrid device for automated transfer of sample preseparated by capillary isoelectric focusing to parallel capillary gel electrophoresis for two-dimensional protein separation.Analytical chemistry · 2012Article
- Protein separation by capillary gel electrophoresis: a review.Analytica chimica acta · 2012Review
- Membrane-assisted online renaturation for automated microfluidic lectin blotting.Journal of the American Chemical Society · 2011Article
- Chemical modification of proteins to improve the accuracy of their relative molecular mass determination by electrophoresis.Electrophoresis · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) is a fundamental analytical technique for proteomic research, and SDS-capillary gel electrophoresis (CGE) is its miniaturized version. Compared to conventional slab-gel electrophoresis, SDS-CGE has many advantages such as increased separation efficiency, reduced separation time, and automated operation. SDS-CGE is not widely accepted in proteomic research primarily due to the difficulties in identifying the well-resolved proteins. MALDI-TOF-MS is an outstanding platform for protein identifications. Coupling the two would solve the problem but is extremely challenging because the MS detector has no access to the SDS-CGE-resolved proteins and the SDS interferes with MS detection. In this work we introduce an approach to address these issues. We discover that poly(tetrafluoroethylene) (PTFE) membranes are excellent materials for collecting SDS-CGE-separated proteins. We demonstrate that we can wash off the SDS bound to the collected proteins and identify these proteins on-membrane with MALDI-TOF-MS. We also show that we can immunoblot and Coomassie-stain the proteins collected on these membranes.
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