ArticleOtology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology2011
Murine middle ear inflammation and ion homeostasis gene expression.
Article in Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Trans-cortical vessels in the mouse temporal bulla bone are a means to recruit myeloid cells in chronic otitis media and limit peripheral leukogram changes.Frontiers in genetics · 2022Article
- Moderate noise associated oxidative stress with concomitant memory impairment, neuro-inflammation and neurodegeneration.Brain, behavior, & immunity - health · 2020Article
- Decreased Aquaporin 4 and 6 mRNAs in Patients With Chronic Otitis Media With Otorrhea.Clinical and experimental otorhinolaryngology · 2019Article
- A Review: Expression of Aquaporins in Otitis Media.International journal of molecular sciences · 2017Review
- Correlative mRNA and protein expression of middle and inner ear inflammatory cytokines during mouse acute otitis media.Hearing research · 2015Article
- Changes in Gene Expression and Hearing Thresholds After Cochlear Implantation.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2015Article
- Intratympanic Steroid Treatments May Improve Hearing via Ion Homeostasis Alterations and Not Immune Suppression.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2015Article
- Control of middle ear inflammatory and ion homeostasis genes by transtympanic glucocorticoid and mineralocorticoid treatments.PloS one · 2015Article
- NOD2/RICK-dependent β-defensin 2 regulation is protective for nontypeable Haemophilus influenzae-induced middle ear infection.PloS one · 2014Article
- Inner ear tissue remodeling and ion homeostasis gene alteration in murine chronic otitis media.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2013Article
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- Otitis media: molecular impact of inflammation in the middle and inner ear--cytokines, steroids, and ion homeostasis.The Laryngoscope · 2012Article
- Cytokine responses in the common cold and otitis media.Current allergy and asthma reports · 2012Review
- Article
- Tissue remodeling in the acute otitis media mouse model.International journal of pediatric otorhinolaryngology · 2011Article
- Ion homeostasis in the ear: mechanisms, maladies, and management.Current opinion in otolaryngology & head and neck surgery · 2010Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
hypothesisIon homeostasis genes are responsible for the movement of ions and water in the epithelium of the middle ear.
backgroundIt is not well known to what extent disruption of ion homeostasis is a factor in the accumulation of middle ear fluid during otitis media.
methodsBalb/c mice were transtympanically injected with heat-killed Hemophilus influenza bacteria. Untreated and saline-injected mice were used as controls. Mice were euthanized at 6, 24, and 72 hours and 1 week after injection, the bullae harvested, and total ribonucleic acid isolated from the middle ear tissues. Ion homeostasis genes were analyzed with real-time quantitative reverse transcription-polymerase chain reaction from the following gene families: Na,K-ATPase, claudins, K transport channels, epithelial Na channels, gap junctions, and aquaporins. Inflammatory genes also were analyzed to document inflammation.
resultsAll inflammatory genes analyzed were significantly upregulated, more at 6 hours than at 24 hours, with the exception of vascular endothelial growth factor and Mapk8. Most middle ear ion homeostasis genes experienced downregulation because of inflammation. This was most prominent in the aquaporin and Na,K-ATPase genes. Significant upregulation was seen in several genes in response to inflammation and saline independently.
conclusionThe innate immune response to bacteria in the middle ear induces expression of several inflammatory genes. Coinciding with this inflammation is the downregulation of numerous ion homeostasis genes that are involved in ion and water transport and maintenance of tight junctions. This may explain the fluid accumulation within the middle ear seen with both acute and chronic otitis media.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.