ArticleMolecular biology of the cell2011
The posttranslational modification of tubulin undergoes a switch from detyrosination to acetylation as epithelial cells become polarized.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Emerging Roles of Tubulin Isoforms and Their Post-Translational Modifications in Microtubule-Based Transport and Cellular Functions.Biomolecules · 2026Review
- Enhancing intestinal epithelial microtubule stability could alleviate IBD symptoms.Cell communication and signaling : CCS · 2025Article
- Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.Cell and tissue research · 2025Article
- Host-Parasite Interactions inInternational journal of molecular sciences · 2024Article
- Altered Posttranslational Modification of Microtubules Contributes to Disturbed Enterocyte Morphology in Celiac Disease.International journal of molecular sciences · 2023Article
- Mutational inactivation of Apc in the intestinal epithelia compromises cellular organisation.Journal of cell science · 2021Article
- TTL-Expression Modulates Epithelial Morphogenesis.Frontiers in cell and developmental biology · 2021Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Improved In Vitro Model for Intranasal Mucosal Drug Delivery: Primary Olfactory and Respiratory Epithelial Cells Compared with the Permanent Nasal Cell Line RPMI 2650.Pharmaceutics · 2019Article
- Cell polarization: From epithelial cells to odontoblasts.European journal of cell biology · 2019Review
- Coordination of microtubule acetylation and the actin cytoskeleton by formins.Cellular and molecular life sciences : CMLS · 2018Review
- Microtubule Motors in Establishment of Epithelial Cell Polarity.Cold Spring Harbor perspectives in biology · 2018Review
- Extracellular matrix stiffness and cell contractility control RNA localization to promote cell migration.Nature communications · 2017Article
- Intracellular Colocalization of Influenza Viral RNA and Rab11A Is Dependent upon Microtubule Filaments.Journal of virology · 2017Article
- DIDO as a Switchboard that Regulates Self-Renewal and Differentiation in Embryonic Stem Cells.Stem cell reports · 2017Article
- Mechanism of microtubule lumen entry for the α-tubulin acetyltransferase enzyme αTAT1.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Altered Hepatic Tubulin Code in Alcoholic Liver Disease.Biomolecules · 2015Review
- Post-translational modifications of tubulin: pathways to functional diversity of microtubules.Trends in cell biology · 2015Review
- Nitrosative stress triggers microtubule reorganization in Arabidopsis thaliana.Journal of experimental botany · 2014Article
- TIG3: an important regulator of keratinocyte proliferation and survival.The Journal of investigative dermatology · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tubulin posttranslational modifications (PTMs) have been suggested to provide navigational cues for molecular motors to deliver cargo to spatially segregated subcellular domains, but the molecular details of this process remain unclear. Here we show that in Madin-Darby Canine Kidney (MDCK) epithelial cells, microtubules express several tubulin PTMs. These modifications, however, are not coordinated, and cells have multiple subpopulations of microtubules that are marked by different combinations of PTMs. Furthermore these subpopulations show differential sensitivity to both drug- and cold-induced depolymerization, suggesting that they are functionally different as well. The composition and distribution of modified microtubules change as cells undergo the morphogenesis associated with polarization. Two-dimensionally polarized spreading cells have more detyrosinated microtubules that are oriented toward the leading edge, but three-dimensionally polarized cells have more acetylated microtubules that are oriented toward the apical domain. These data suggest that the transition from 2D polarity to 3D polarity involves both a reorganization of the microtubule cytoskeleton and a change in tubulin PTMs. However, in both 2D polarized and 3D polarized cells, the modified microtubules are oriented to support vectorial cargo transport to areas of high need.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.