ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Control of breathing by raphe obscurus serotonergic neurons in mice.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed.
- Intrinsic pH chemosensitivity of acutely dissociated medullary serotonergic and retrotrapezoid neurones.The Journal of physiology · 2026Article
- Noradrenergic input to hypoglossal motoneurons is mediated by interneurons located in prehypoglossal region.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- New molecular markers to differentiate carbon dioxide intoxication from asphyxia due to oxygen deficiency.Forensic science, medicine, and pathology · 2025Article
- Review
- Functional modulation of retrotrapezoid neurons drives fentanyl-induced respiratory depression.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Early life stress and hormonal status influence orexin-1 receptor expression in structures regulating cardiorespiratory responses to COExperimental physiology · 2025Article
- Article
- Asymmetric neuromodulation in the respiratory network contributes to rhythm and pattern generation.Frontiers in neural circuits · 2025Article
- Inhibiting cholinergic signalling in the cerebellar interpositus nucleus impairs motor behaviour.The European journal of neuroscience · 2024Article
- Revisiting the Role of Serotonin in Sleep-Disordered Breathing.International journal of molecular sciences · 2024Review
- Leptin signaling in the dorsomedial hypothalamus couples breathing and metabolism in obesity.Cell reports · 2023Article
- Respiration organizes gamma synchrony in the prefronto-thalamic network.Scientific reports · 2023Article
- Altered 5-HT2A/C receptor binding in the medulla oblongata in the sudden infant death syndrome (SIDS): Part I. Tissue-based evidence for serotonin receptor signaling abnormalities in cardiorespiratory- and arousal-related circuits.Journal of neuropathology and experimental neurology · 2023Article
- Review
- Transformation of Our Understanding of Breathing Control by Molecular Tools.Annual review of physiology · 2023Review
- Article
- Criteria for central respiratory chemoreceptors: experimental evidence supporting current candidate cell groups.Frontiers in physiology · 2023Review
- Serotonin and the ventilatory effects of etonogestrel, a gonane progestin, in a murine model of congenital central hypoventilation syndrome.Frontiers in endocrinology · 2023Article
- Could respiration-driven blood oxygen changes modulate neural activity?Pflugers Archiv : European journal of physiology · 2023Review
- Effects of Prenatal Cannabinoids Exposure upon Placenta and Development of Respiratory Neural Circuits.Advances in experimental medicine and biology · 2023Review
40 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
Abstract
We used optogenetics to determine the global respiratory effects produced by selectively stimulating raphe obscurus (RO) serotonergic neurons in anesthetized mice and to test whether these neurons detect changes in the partial pressure of CO(2), and hence function as central respiratory chemoreceptors. Channelrhodopsin-2 (ChR2) was selectively (∼97%) incorporated into ∼50% of RO serotonergic neurons by injecting AAV2 DIO ChR2-mCherry (adeno-associated viral vector double-floxed inverse open reading frame of ChR2-mCherry) into the RO of ePet-Cre mice. The transfected neurons heavily innervated lower brainstem and spinal cord regions involved in autonomic and somatic motor control plus breathing but eschewed sensory related regions. Pulsed laser photostimulation of ChR2-transfected serotonergic neurons increased respiratory frequency (fR) and diaphragmatic EMG (dEMG) amplitude in relation to the duration and frequency of the light pulses (half saturation, 1 ms; 5-10 Hz). dEMG amplitude and fR increased slowly (half saturation after 10-15 s) and relaxed monoexponentially (tau, 13-15 s). The breathing stimulation was reduced ∼55% by methysergide (broad spectrum serotonin antagonist) and potentiated (∼16%) at elevated levels of inspired CO(2) (8%). RO serotonergic neurons, identified by their entrainment to short light pulses (threshold, 0.1-1 ms) were silent (nine cells) or had a low and regular level of activity (2.1 ± 0.4 Hz; 11 cells) that was not synchronized with respiration. These and nine surrounding neurons with similar characteristics were unaffected by adding up to 10% CO(2) to the breathing mixture. In conclusion, RO serotonergic neurons activate breathing frequency and amplitude and potentiate the central respiratory chemoreflex but do not appear to have a central respiratory chemoreceptor function.
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