ArticleThe Journal of general virology2011
Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production.
Article in The Journal of general virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 146 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
146 citing papers in PubMed, 298 citations in OpenAlex.
- Research progress on NSP4 protein of porcine reproductive and respiratory syndrome virus.Veterinary research communications · 2026Review
- Computational modelling of the equine arteritis virus GP5/M Dimer: Implications for immune evasion and virulence.PloS one · 2026Article
- Development of a TaqMan probe-based dual real time PCR assay for the identification of NADC34-like PRRSV.Veterinary and animal science · 2025Article
- The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoating.Journal of virology · 2025Article
- Refining Lineage Classification and Updated RFLP Patterns of PRRSV-2 Revealed Viral Spatiotemporal Distribution Characteristics in China in 1991-2023.Transboundary and emerging diseases · 2025Article
- Isolation and pathogenicity of the novel porcine reproductive and respiratory syndrome virus 1 strain ZJ01 in South China.Frontiers in cellular and infection microbiology · 2025Article
- The role of major and minor structural proteins of porcine reproductive and respiratory syndrome virus in induction of protective immunity.Frontiers in microbiology · 2025Article
- Recombination of Porcine Reproductive and Respiratory Syndrome Virus: Features, Possible Mechanisms, and Future Directions.Viruses · 2024Review
- Fidelity Characterization of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and NADC30-like Strain.Viruses · 2024Article
- GTPase activity of porcine Mx1 plays a dominant role in inhibiting the N-Nsp9 interaction and thus inhibiting PRRSV replication.Journal of virology · 2024Article
- Construction and immunological evaluation of recombinant adenovirus vaccines of new novel NADC34-PRRSV strains in pigs.Frontiers in veterinary science · 2024Article
- Sialoadhesin-dependent susceptibility and replication of porcine reproductive and respiratory syndrome viruses in CD163-expressing cells.Frontiers in veterinary science · 2024Article
- Genetic variation and recombination analysis of theFrontiers in veterinary science · 2024Article
- Potential SLA Hp-4.0 haplotype-restricted CTL epitopes identified from the membrane protein of PRRSV induce cell immune responses.Frontiers in microbiology · 2024Article
- Article
- Recent Progress in Studies of Porcine Reproductive and Respiratory Syndrome Virus 1 in China.Viruses · 2023Review
- The rPRRSV-E2 strain exhibited a low level of potential risk for virulence reversion.Frontiers in veterinary science · 2023Article
- Epidemiological investigation and pathogenicity of porcine reproductive and respiratory syndrome virus in Sichuan, China.Frontiers in microbiology · 2023Article
- Isolation, Pathogenicity, and Comparative Phylogenetic Characteristics of an Intralineage Recombinant NADC34-Like PRRSV in China.Transboundary and emerging diseases · 2023Article
- Article
86 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
The arterivirus family (order Nidovirales) of single-stranded, positive-sense RNA viruses includes porcine respiratory and reproductive syndrome virus and equine arteritis virus (EAV). Their replicative enzymes are translated from their genomic RNA, while their seven structural proteins are encoded by a set of small, partially overlapping genes in the genomic 3'-proximal region. The latter are expressed via synthesis of a set of subgenomic mRNAs that, in general, are functionally monocistronic (except for a bicistronic mRNA encoding the E and GP2 proteins). ORF5, which encodes the major glycoprotein GP5, has been used extensively for phylogenetic analyses. However, an in-depth computational analysis now reveals the arterivirus-wide conservation of an additional AUG-initiated ORF, here termed ORF5a, that overlaps the 5' end of ORF5. The pattern of substitutions across sequence alignments indicated that ORF5a is subject to functional constraints at the amino acid level, while an analysis of substitutions at synonymous sites in ORF5 revealed a greatly reduced frequency of substitution in the portion of ORF5 that is overlapped by ORF5a. The 43-64 aa ORF5a protein and GP5 are probably expressed from the same subgenomic mRNA, via a translation initiation mechanism involving leaky ribosomal scanning. Inactivation of ORF5a expression by reverse genetics yielded a severely crippled EAV mutant, which displayed lower titres and a tiny plaque phenotype. These defects, which could be partially complemented in ORF5a-expressing cells, indicate that the novel protein, which may be the eighth structural protein of arteriviruses, is expressed and important for arterivirus infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.