ArticleJournal of clinical microbiology2011
Detection of hemagglutinin variants of the pandemic influenza A (H1N1) 2009 virus by pyrosequencing.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- New insights into the neuraminidase-mediated hemagglutination activity of influenza A(H3N2) viruses.Antiviral research · 2023Article
- Agnostic Sequencing for Detection of Viral Pathogens.Clinical microbiology reviews · 2023Review
- Detection of baloxavir resistant influenza A viruses using next generation sequencing and pyrosequencing methods.Antiviral research · 2020Article
- Molecular and Immunological Diagnostic Techniques of Medical Viruses.International journal of microbiology · 2020Review
- A Pyrosequencing-Based Approach to High-Throughput Identification of Influenza A(H3N2) Virus Clades Harboring Antigenic Drift Variants.Journal of clinical microbiology · 2017Article
- Enhanced Genetic Characterization of Influenza A(H3N2) Viruses and Vaccine Effectiveness by Genetic Group, 2014-2015.The Journal of infectious diseases · 2016Article
- Review
- Deep sequencing: becoming a critical tool in clinical virology.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2014Review
- The effect of the MDCK cell selected neuraminidase D151G mutation on the drug susceptibility assessment of influenza A(H3N2) viruses.Antiviral research · 2014Article
- Article
- Hemagglutinin 222D/G polymorphism facilitates fast intra-host evolution of pandemic (H1N1) 2009 influenza A viruses.PloS one · 2014Article
- Rapid strategy for screening by pyrosequencing of influenza virus reassortants--candidates for live attenuated vaccines.PloS one · 2014Article
- Cell culture-selected substitutions in influenza A(H3N2) neuraminidase affect drug susceptibility assessment.Antimicrobial agents and chemotherapy · 2013Article
- Effect of oseltamivir carboxylate consumption on emergence of drug-resistant H5N2 avian influenza virus in Mallard ducks.Antimicrobial agents and chemotherapy · 2013Article
- Article
- Assays for monitoring susceptibility of influenza viruses to neuraminidase inhibitors.Influenza and other respiratory viruses · 2013Review
- Neuraminidase H275Y and hemagglutinin D222G mutations in a fatal case of 2009 pandemic influenza A (H1N1) virus infection.Influenza and other respiratory viruses · 2012Article
- A new look at an old virus: patterns of mutation accumulation in the human H1N1 influenza virus since 1918.Theoretical biology & medical modelling · 2012Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For influenza viruses, pyrosequencing has been successfully applied to the high-throughput detection of resistance markers in genes encoding the drug-targeted M2 protein and neuraminidase. In this study, we expanded the utility of this assay to the detection of multiple receptor binding variants of the hemagglutinin protein of influenza viruses directly in clinical specimens. Specifically, a customized pyrosequencing protocol that permits detection of virus variants with the D, G, N, or E amino acid at position 222 in the hemagglutinin of the 2009 pandemic influenza A (H1N1) virus was developed. This customized pyrosequencing protocol was applied to the analysis of 241 clinical specimens. The use of the optimized nucleotide dispensation order allowed detection of mixtures of variants in 10 samples (4.1%) which the standard cyclic nucleotide dispensation protocol failed to detect. The optimized pyrosequencing protocol is expected to provide a more accurate tool in the analysis of virus variant composition.
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