ArticleDevelopment (Cambridge, England)2011
Kinesin-1 tail autoregulation and microtubule-binding regions function in saltatory transport but not ooplasmic streaming.
Article in Development (Cambridge, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Sequences in the stalk domain regulate auto-inhibition and ciliary tip localization of the immotile kinesin-4 KIF7.Journal of cell science · 2021Article
- Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in theMolecular biology of the cell · 2020Article
- Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts.The Journal of cell biology · 2018Article
- Microtubule-microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- JIP3 Activates Kinesin-1 Motility to Promote Axon Elongation.The Journal of biological chemistry · 2015Article
- Unique function of Kinesin Kif5A in localization of mitochondria in axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Human CFEOM1 mutations attenuate KIF21A autoinhibition and cause oculomotor axon stalling.Neuron · 2014Article
- The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline.Development (Cambridge, England) · 2014Article
- The molecular basis for kinesin functional specificity during mitosis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- Loss of syd-1 from R7 neurons disrupts two distinct phases of presynaptic development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Kinesin tail domains are intrinsically disordered.Proteins · 2012Article
- Three routes to suppression of the neurodegenerative phenotypes caused by kinesin heavy chain mutations.Genetics · 2012Article
- Unconventional functions of microtubule motors.Archives of biochemistry and biophysics · 2012Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The N-terminal head domain of kinesin heavy chain (Khc) is well known for generating force for transport along microtubules in cytoplasmic organization processes during metazoan development, but the functions of the C-terminal tail are not clear. To address this, we studied the effects of tail mutations on mitochondria transport, determinant mRNA localization and cytoplasmic streaming in Drosophila. Our results show that two biochemically defined elements of the tail - the ATP-independent microtubule-binding sequence and the IAK autoinhibitory motif - are essential for development and viability. Both elements have positive functions in the axonal transport of mitochondria and determinant mRNA localization in oocytes, processes that are accomplished by biased saltatory movement of individual cargoes. Surprisingly, there were no indications that the IAK autoinhibitory motif acts as a general downregulator of Kinesin-1 in those processes. Time-lapse imaging indicated that neither tail region is needed for fast cytoplasmic streaming in oocytes, which is a non-saltatory bulk transport process driven solely by Kinesin-1. Thus, the Khc tail is not constitutively required for Kinesin-1 activation, force transduction or linkage to cargo. It might instead be crucial for more subtle elements of motor control and coordination in the stop-and-go movements of biased saltatory transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.